H2020Индивидуална стипендия2019–2021

Upbiosens · Near-infrared nucleic acids sensing and imaging using lanthanide-based nanoparticles capped with DNA

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2019-05-01 → 2021-04-30
Финансиране от ЕС: 185 076 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Наносензори от специални частици и нишки ДНК се разработват за откриване и визуализиране на генетичен материал, като например микро-РНК. Те могат да помогнат за по-бързата и точна диагностика на заболявания като рака.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Near-infrared nucleic acids sensing and imaging using lanthanide-based nanoparticles capped with DNA

Biosensing refers to the use of a biological derived or bioinspired receptor to detect the presence of a particular analyte with high specificity. The development of highly sensitive and specific nucleic acids (NA), i.e., DNA or RNA, biosensors has received increasing attention due to their crucial role in diagnostic and therapeutic applications (e.g., detection of disease biomarkers). Especially, the development of multiplexed micro-RNA sensors (miRNA, small non-coding RNAs) due to the role of miRNAs as regulators of gene expression. Overexpression levels can be associated with human diseases such as cancer and today, miRNAs are so-called next-generation biomarkers. Despite the development of methods to analyze NA, such as polymerase chain reaction (PCR), some technical shortcomings still hinder an easy-to-use, reproducible, storable, rapid, sensitive, specific, versatile, and multiplexed NA detection. Due to the near-infrared (NIR) excitation, multiple and narrow emission bands over a broad wavelength range, and lack of photoblinking and photobleaching, the use of upconversion nanoparticles (UCNPs) offers the opportunity to design highly sensitive multiplexed UCNPs-based sensing probes. The Upbiosens project describes a novel method to produce a nanobiosensor combining the sensing ability of the DNA strands and the optical properties of the UCNPs as the transducer element to detect and visualize DNA or/and miRNA. Hence, the project addresses the problem of capping UCNPs with DNA while keeping their hybridization capacity as well as controlling the UCNP size, overcoming one of the main limitations of the UCNPs for their application as NA sensors. The outcome of the project can be promisingly bio-implemented, such as in prognosis, diagnostics, and treatment of diseases, gene therapy, or forensic analysis.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The Upbiosens project describes a novel method to produce water-dispersible and stable DNA-capped upconversion nanoparticles (UCNPs), keeping intact (or enhancing) the properties of both DNA and UCNPs. The UCNP@DNA nanohybrid will be able to detect the complementary target miRNA or DNA sequence. This detection will be based on the UCNP emission changes after NIR excitation in the presence of the fluorophore target RNA/DNA sequence. The hybridization will enable FRET from the UCNP to the fluorophore upon NIR irradiation, which will allow the quantitative measurement of nucleic acid. In addition, this new strategy for DNA functionalization of UCNPs will enable the control of the UCNP size during the functionalization step as well as the distance between UCNP and the energy acceptor. Both issues are critical for the design of novel, rapid, highly selective and sensitive FRET-based biosensors. This approach will be demonstrated for wo different compositions of UCNPs, NaYF4: Yb, Er (excitation at 980 nm) and NaYF4: Yb, Nd, Er (excitation at 800 nm) in order to enhance optical properties. The excitation at 800 nm reduces the overheating of biosamples due to the light absorption by water. Taking into account the exceptional UCNPs emissive properties, the capability for in vitro nucleic acid imaging of the nanobiosensor will also be evaluated. To our knowledge, the combination of a nucleic acid sensing and imaging using UCNP-based nanohybrids has not yet been attempted. This dual NIR NA biosensor/bioimaging nanohybrid can be promisingly bio-implemented, such as in prognosis, diagnostics and treatment of diseases, gene therapy, or forensic analysis.

Оригинален текст от CORDIS (на английски).

Участници

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS · ParisКоординаторФранция