H2020Индивидуална стипендия2018–2020

THALLMORPHAL · Insight into the Symbiotic Chemical Communication of Algae and Bacteria: Thallusin and Dedicated Analogues

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-09-01 → 2020-08-31
Финансиране от ЕС: 159 461 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Химическата комуникация между водорасли и бактерии се изучава чрез синтезиране на веществото талазин, което стимулира растежа на структури като листа и корени. Това помага за разбирането на развитието на водораслите от род Ulva, чието прекомерно размножаване вреди на морските екосистеми.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Insight into the Symbiotic Chemical Communication of Algae and Bacteria: Thallusin and Dedicated Analogues

Thallusin is a terpenoid hybrid that triggers the growth of leaf-like structure in alga Monostroma oxyspermum at a concentration as low as 10-15 g/L. Thallusin is produced by the bacteria that live in a symbiotic relationship with algae in their natural habitat. However, its general impact on the underlying communities and its mode of actions remain to be clarified. Recently, it has been demonstrated that thallusin induces the rhizoid and cell wall formation in Ulva mutabilis in the presence of Roseovarius sp. bacteria. Compared to Monostroma, thallusin has a different function in Ulva. It induced cell differentiation and formation of the holdfast organ < 10-10 g/L concentrations. The genus Ulva has a profound economic and scientific value. It grows mainly in intertidal zones. Eutrophication of coastal waters results in the rapid growth of such species, significantly increasing their biomass and thus forming, e.g., green tides, which leads to the impairment of the coastal marine ecosystem. Access to thallusin is highly essential for biological experiments as its retrieval from natural sources is difficult. For biological work, the only isolated material in µ quantities has been used so far, so data beyond mass spectrometry was challenging to obtain. We, therefore, aimed at developing a synthetic route to thallusin, which enables scalable access to thallusin and its derivatives that can be used as a tool for studying the morphogenesis in Ulva. A 6-endo-cyclization was envisioned for the one-pot assembly of the pyridine substituted dihydropyran ring-system found in thallusin. Synthetic thallusin, its analogs, and labeled tool compounds were envisioned to significantly contribute to a better understanding of thallusin activity and relevance for algae growth. At last, data collected from all the above-described experiments will be crucial for identifying the mode-of-action and yet-unknown biosynthesis of naturally occurring thallusin. In the long term, these data will collectively stimulate our understanding of the growth of macroalgae, that have the highest relevance for carbon fixation in marine ecosystems, as well as for using algae in the production of chemicals and for biotechnology.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Thallusin was the first compound identified to induce thallus differentiation in macroalgae. This intriguing chemical mediator is produced by bacteria that socialize with algae in their natural habitat. Thallusin exemplifies a fundamental symbiotic chemical communication between macroalgae and epiphytic bacteria in the marine environment. However, its general impact on the underlying microbial communities and its chemical mode-of-action remains to be clarified. Therefore we propose a multifaceted research program to study the morphogenesis-inducing mechanism of algae by chemical morphogen. The proposed project will focus at four different objectives. 1) methodology development (heteroarylative/cyclization cascade), that allows the efficient and concise synthesis of thallusin. This will be achieved by ligand directed transition metal catalysis based on good literature precedent. The first objective is scheduled to be completed in first six month of the project. 2) development of novel, concise, scalable, and efficient asymmetric synthesis of (‒)-thallusin and its analogues. This will be accomplished by enantioselective polyene cyclization and heteroarylative/cyclization cascade as key steps. The second objective is scheduled to be completed from months 6-12 and 17-20. 3) structure-activity- relationships studies and 4) understanding the mode-of-action of thallusin in algae. The objective 3 and 4 will be completed in collaborations. The labeled tool compounds and dye conjugates will be synthesized from promising candidates that will allow tracing the metabolism and degradation of thallusin in algae. The 3 and 4 objective is scheduled to be completed from 9-24 months. The results that will be obtained during the course of our research will have the potential capacity to enlighten the cross-kingdom cell-to-cell chemical signaling and will provide room for further studies at the fundamental and applied levels in green algae and symbiotic bacteria association.

Оригинален текст от CORDIS (на английски).

Участници

FRIEDRICH-SCHILLER-UNIVERSITÄT JENA · JENAКоординаторГермания