H2020Индивидуална стипендия2018–2020

NeuroVU · Real-time Sensing in Microfluidic Models of the Neurovascular Unit

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-06-27 → 2020-06-26
Финансиране от ЕС: 185 857 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Невроваскуларната единица се изследва чрез създаване на микрофлуидни модели „орган на чип“, които следят биохимичните процеси в реално време. Това помага за по-доброто разбиране на химията в мозъка и ускорява разработването на нови лекарства за централната нервна система.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

NeuroVU: Real-time Sensing in Microfluidic Models of the Neurovascular Unit

Brain-related disorders and diseases account for ~45 million disability-adjusted life-years (DALYs) lost, or around 15% of the total, in Europe alone. While this number is projected to rise, development of drugs for the central nervous system (CNS) has slowed down significantly over the past decades. Among the major challenges identified are the comparatively poor existing model systems (i.e., in-vitro cell-based models and rodents) and the resulting shortcomings in our understanding of brain biochemistry. Of particular interest for delivery of therapeutics to the CNS is the neurovascular unit (NVU), the interface between systemic circulation and the CNS. The aim of my research project was to find a holistic solution for integration of biophysical and biochemical sensing capabilities, microfluidics, and neurovascular cells to provide a novel class of organ-on-a-chip (OoC) models of the neurovascular unit (NVU) with advanced real-time sensing capabilities, termed NeuroVU. These systems have the potential to provide unprecedented insight into neurovascular chemistry as well as accelerate pharmaceutical development. The research was carried out at the Kungliga Tekniska Högskolan (KTH) Stockholm in the Department of Micro- and Nanosystems (MST) under the supervision of Professor Anna Herland.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Disorders of the central nervous system are one of the grand health challenges of this century. Therapeutic development, however, remains limited by poor understanding of the brain, including of the neurovascular unit (NVU). The NVU acts as the gatekeeper between blood and brain for metabolites, disease agents, as well as drugs. Much research has relied on animals or simple cell models that recapitulate neither the cellular ensemble nor the environment of the NVU. Emerging Organ-on-Chip microsystems promise to overcome these limitations, but to date fall short on the relevance of cells used and the lack of continuous monitoring capabilities for chemical markers, metabolic conversions, and pharmacodynamics.My objective in this project is to develop the first NVU Organ-on-Chip integrating human-normal cells as well as biophysical and biochemical sensors for real-time monitoring, termed NeuroVU. I propose to rely on primary and induced pluripotent stem cells, which can achieve in-vivo-like properties, and off-stoichiometry thiol-ene-epoxies (OSTE+) as the enabling technologies. OSTE+ is a versatile polymer uniquely suited to supersede the prevalent polydimethylsiloxane/glass paradigm for the innovative system integration approaches needed here. I will first characterize and optimize it for compatibility with biological and sensor components. I will build on these component insights with microsystems integration to develop NeuroVUs featuring co-culture of NVU-normal cells, continuous monitoring of relevant ions & metabolites in addition to trans-endothelial electrical resistance, and tissue-like hydrogel structures. I will leverage collaborations with resident experts in materials and biology as well as my own strong background in microsystems integration to realize this highly interdisciplinary project. Ultimately, the NeuroVU will allow for unprecedented insights on NVU chemistry and has the potential to significantly accelerate pharmaceutical development.

Оригинален текст от CORDIS (на английски).

Участници

KUNGLIGA TEKNISKA HOEGSKOLAN · StockholmКоординаторШвеция