H2020Индивидуална стипендия2018–2020

STARTlight · cell cycle START decision unraveled by single-cell microscopy, modeling and optogenetics

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-04-01 → 2020-03-31
Финансиране от ЕС: 165 599 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Молекулярните механизми, чрез които единични клетки на дрожди решават кога да започнат делене, се анализират чрез микроскопия и математическо моделиране. Разбирането на този процес помага да се разбере как се развива ракът при хората поради нарушеното клетъчно делене.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

STARTlight: cell cycle START decision unraveled by single-cell microscopy, modeling and optogenetics

Cells across all kingdoms of life need to maintain tight control on their division process, which they use to replicate themselves. In particular, the decision of cells to initiate a round of division is a ubiquitous and essential process, whose dysregulation can dramatically affect cell viability in simple unicellular organisms, while contributing to carcinogenesis in organisms such as humans. The central goal of this proposal was to determine the key molecular mechanisms behind this decision process. To achieve this, we used a combination of cutting-edge experimental methods with data analysis and mathematical modeling to understand how and when individual budding yeast cells decide to divide. Our results have generated new fundamental knowledge on the regulation of the yeast cell division cycle which, thanks to the high degree of evolutionary conservation of cell division mechanisms among different organisms, may also be applicable to organisms such as humans.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Cells across all kingdoms of life have developed exquisite control mechanisms to achieve and maintain the balance between cell growth and division. The decision to commit to cell division is a ubiquitous and essential process, whose dysregulation can dramatically affect cell viability in simple eukaryotes, while leading to tumorigenesis in mammalian cells. In budding yeast, the short interval in late G1 where commitment to cell division is decided, is known as START. Despite years of research, it is still unclear how the transition through START is triggered, and many mutually conflicting hypotheses have been proposed. Recent developments in microfluidics and single-cell microscopy have opened up new avenues for investigating START at the single-cell level. Moreover, thanks to the rapid development of optogenetic tools in the last few years offers the possibility to apply dynamic single-cell perturbations, with great potential for untangling complex intracellular networks. By combining single-cell time-course data on key components of the START network with targeted, reversible and dynamic perturbation of their activities using optogenetic tools, the aim of this proposal is to unravel the mechanisms that trigger START in budding yeast and to quantitatively describe these processes in a mathematical model. The results of this project will thus provide new fundamental knowledge on cell cycle regulation which, thanks to the high degree of conservation between yeast and higher eukaryotes, will be transferrable to other organisms as well.

Оригинален текст от CORDIS (на английски).

Участници

RIJKSUNIVERSITEIT GRONINGEN · GroningenКоординаторНидерландия