H2020Индивидуална стипендия2018–2020

INTERSPREC · Investigation of interspecific pollen tube reception

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-07-01 → 2020-06-30
Финансиране от ЕС: 175 420 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Механизмите за разпознаване на прашният прашец се изучават чрез два близки вида растения от род Arabidopsis. Това помага да се разбере как се формират репродуктивните бариери, които разделят различните видове по време на тяхната еволюция.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Investigation of interspecific pollen tube reception

As new species evolve during incipient speciation, different crossing barriers are formed, which prevent the formation of potentially unviable or sterile hybrid offspring. It has been considered that these barriers are weak at the beginning of the split of two species, still allowing the formation of hybrids, albeit this may be rare. Later, stronger barriers evolve that prevent any crossing and the two species are reproductively isolated. We study how speciation emerges, looking at the origin of reproductive barriers. As tool we use two closely related species, Arabidopsis thaliana and Arabidopsis lyrata, where reproductive barriers are still in formation. Depending on the A.thaliana accession, A.lyrata pollen is recognized with a variable degree of success (between 10% and 90%). If recognition does not occur, the pollen tube (PT) is not properly received, keeps growing inside the ovule, and fertilization fails. This recognition step takes place in the cells at the contact point between the embryo sac and PT, the synergids, and can be considered the point of emergence of a first reproductive barrier. Genes involved in reproductive barriers are expected to show rapid divergence. The work focuses on the regulation of the genes found to be important for interspecific PT recognition by the female gametophyte, as well as the molecular characterization of the recognition mechanism. The proposed research project main objectives are: 1. Characterization of the newly discovered gene: generating additional alleles (CRISPR-Cas9), addressing redundancy in synergids, manipulation by ectopic expression 2. Identification of the promoter region of discovered gene from different Arabidopsis species 3. Identification of the factors regulating discovered gene in A. thaliana 4. Training, active dissemination, publication

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Cell-cell interaction mechanisms play a role in many fundamental biological processes from symbiotic interactions of fungi and bacteria with their host plants to fertilization both in animals and plants. In plant reproduction, pollen tube (PT) reception is a cell-cell communication process allowing the recognition of the male gametophyte (the PT) by the female gametophyte necessary for fertilization. To investigate this process, the host lab used interspecific crosses between certain Arabidopsis thaliana accessions and A.lyrata pollen, where most PTs are not recognized, causing a failure in sperm release and thus fertilization. In contrast, several other A.thaliana accessions almost perfectly recognize interspecific PTs. Preliminary results identified the gene At5g62150 as a key player for species-specific PT recognition. At5g62150 codes for a LysM-domain containing glycan-binding peptide, pointing at glycosylation patterns for the distinction of intra- and interspecific PTs, a mechanism already known to be important in symbiosis and immunity. It was found that differences in the regulation of At5g62150 in distinct species is responsible for recognition, as the amino acid sequence does not differ between A. thaliana other accessions. With this proposal, we will first attest and manipulate the expression profile of At5g62150 in different accessions to correlate its regulation with its function. Then, we propose to dissect the regulation of At5g62150 in different ways: identification and study of the essential cis-regulatory element for regulation and Y1H screening and Reverse ChIP to find trans-acting regulators. The output will not only pave the way for the understanding and manipulating crosses barriers in plants, which is highly relevant for plant breeding, and shed light on cell-cell communication processes in general, but also will establish a novel, powerful instrument (Reverse ChIP) to investigate DNA-protein interaction in complex organisms.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITAT ZURICH · ZurichКоординаторШвейцария