PAERADOX · Precursors to Modern Aerobic Ammonium Oxidation: Oxygenic and Electrogenic Anaerobic Pathways
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2018-08-01 → 2022-03-26
- Финансиране от ЕС
- 278 228 €
- Участници
- 2
- Схема
- MSCA-IF
Линиите свързват координатора с партньорите.
Накратко на български
Древните метаболитни пътища при микробите, като например окисляването на амоняк без кислород, се проследяват и реконструират. Това помага да се разбере как животът е еволюирал заедно с биогеохимичните цикли на Земята и другите планети.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Precursors to Modern Aerobic Ammonium Oxidation: Oxygenic and Electrogenic Anaerobic Pathways
Earth has undergone dramatic changes since the start of life, and among them, the rise of oxygen by Cyanobacteria greatly influenced the evolution of life. The appearance of oxygen-dependent (aerobic) metabolisms shaped the modern biosphere, as oxygen breathing is the dominant metabolism on modern Earth. On the other hand, oxygen-independent (anaerobic) metabolisms are still diverse and even persistent in the genomes of the strictly oxygen-dependent microbes. Can the evolutionary history of microbial metabolisms on our planet be uncovered by research on such persistent oxygen-independent metabolism in modern aerobic microbes? PARAEDOX aims to discover the novel anaerobic microbial metabolisms and the story of ancient microbial metabolisms on Earth. Research on the origins and evolution of microbial metabolisms can ultimately help us explain how and when life co-evolved with biogeochemical cycles and may help to evaluate the state of evolution on other planets. We trace unidentified ancient anaerobic microbial pathways associated with ammonia oxidation and reconstruct the evolutionary history of those metabolisms.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
How did microbes evolve to use O2? How did O2-dependent pathways derive from or evolve under anoxic conditions? This central evolutionary mystery remains essentially unresolved. I contend that it is possible to identify the evolutionary history of modern aerobic pathways (APs), by discovering the vestiges of ancient anaerobic pathways (AnPs) that persist in modern microbes. Aerobic nitrifiers contain aerobic metabolisms, while few clues such as chlorite dismutase enzyme in Nitrospira, NO dismutase enzyme in Methylomirabilis, and electrochemical signals from aerobic nitrifiers support the notion that remnants of AnPs remain. Hence, I postulate that in nitrifiers currently considered strict aerobes, I can identify the vestiges and integral remains of the earliest APs: I will identify the AnPs of NH4+ oxidation that are driven by intracellular O2 production and respiration mediated by EET. By applying BES and batch incubations followed by isotopic and genomic experiments will evidence and characterize the novel AnPs in nitrifiers. Single cell genomics on FAC-sorted fluorescent-tagged cells followed by molecular phylogenetic analysis will provide a major contribution to unravelling the early evolution of aerobiosis and aerobic NH4+ oxidation, which is crucial as we seek the origins of life on Earth, and other planets. PAERADOX will provide me an interdisciplinary research training in the area of microbial physiology and genomics, molecular biology and computational evolution through international mobility (Harvard, MIT and DTU). Such a training-through-research platform will enhance my career development to a large extent. My return and reintegration to DTU will provide basis to transfer my interdisciplinary combination to European scientific community. I am confident that the international network, skills and knowledge that will be constructed during PAERADOX is an excellent investment for my future and for EU’s strength in the field of microbial evolution and ecology.
Оригинален текст от CORDIS (на английски).
Участници
- DANMARKS TEKNISKE UNIVERSITET · Kongens LyngbyКоординаторДания
- PRESIDENT AND FELLOWS OF HARVARD COLLEGE · CambridgeСъединени щати
Връзки
Данни: CORDIS, © Европейски съюз