H2020Индивидуална стипендия2018–2020

ArtMetBio · Directed Evolution of Novel Artificial Metalloenzyme Platforms for Biocatalysis

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2018-05-01 → 2020-04-30
Финансиране от ЕС: 175 420 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Изкуствените металоензими съчетават синтетични метални катализатори с естествени протеини, за да се създадат хибридни системи за химични реакции. Тези платформи помагат за подобряване на ефективността и гъвкавостта на процесите в биохимията и индустрията.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Directed Evolution of Novel Artificial Metalloenzyme Platforms for Biocatalysis

Catalysis is a critical component of biochemistry and modern industry. In both of these fields, the versatility of a coordinated metal ion is frequently key to obtaining the desired catalytic function. In biology, enzymes often recruit metals to catalyze challenging transformations, such as C-H activation, nitrogen fixation, and water splitting. Despite the diversity of these reactions, natural enzymes are limited by the repertoire of bioavailable metals and ligands. In contrast, synthetic chemists can select from a wider variety of metal ions and ligands to create industrial catalysts. Thus, abiological metal catalysts enable reactions not found in nature with high enantioselectivity, versatility, and broad substrate scope. These catalysts, however, are often inferior to enzyme catalysts in terms of turnover number, efficiency, and selectivity. To take advantage of these complementary approaches, artificial metalloenzymes have emerged as a synergistic fusion of synthetic and biological catalysts. These hybrid catalysts have the potential to harness the advantages of both systems, inheriting the versatility of synthetic catalysts and the efficiency and robustness of enzymes. The objective of the ArtMetBio project is to create novel platforms for artificial metalloenzymes that optimize catalytic performance and versatility via increased cooperation between the synthetic and biological components.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Artificial metalloenzymes have emerge as a fusion of synthetic and biological catalysts for environmentally friendly synthesis. These hybrid catalysts are created by tethering a catalytic metal ion or cofactor to a protein scaffold. Optimized artificial metalloenzymes have the potential to harness the advantages of both systems, inheriting the versatility of synthetic catalysts and the efficiency and robustness of enzymes. New tools, however, are required to realize the potential of artificial metalloenzymes. The objective of the ArtMetBio project is to create novel platforms for artificial metalloenzymes that optimize catalytic performance and versatility via increased cooperation between the synthetic and biological components. Now, more than ever, green catalysis is a crucial global endeavor. Our growing ability to evolve enzymes enables the use of new, unexpected protein scaffolds, opening the door to new innovative platforms. Taking advantage of directed evolution methods, this proposal outlines a strategy for repurposing two well characterized protein scaffolds to create artificial metalloenzymes for in vivo metathesis, a canonical reaction in synthetic chemistry not found in nature. Aim 1 of the project is to optimize metathesis-catalyzing enzymes, coined metathases, through directed evolution. Aim 2 will apply these metathases to engineer a biocatalytic cascade for in vivo synthesis of high-value privileged compounds. The proposed systems differ from the current state-of-the art—streptavidin and heme artificial metalloenzymes—because they are monomeric, non-toxic, and highly modular, enabling wide catalytic versatility. The proposed action has the potential to provide unique, versatile tools for synthesis and biocatalysis.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITAT BASEL · BaselКоординаторШвейцария