H2020Индивидуална стипендия2019–2021

MNSWLGM · An optofluidic platform based on liquid-gradient refractive index microlens for the isolation and quantification of extracellular vesicles

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2019-08-01 → 2021-07-31
Финансиране от ЕС: 187 420 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Вънтоклетъчните везикули са малки частици, отделяни от клетките, които се изследват чрез нова оптофлуидна платформа за тяхното отделяне и броене. Това помага за по-лесното идентифициране на биомаркери, използвани при диагностиката и терапията на различни заболявания.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

An optofluidic platform based on liquid-gradient refractive index microlens for the isolation and quantification of extracellular vesicles

Extracellular vesicles (EVs) describe distinct populations of small (30-200 nm) and large (500 nm-2 μm) microvesicles actively or passively secrected by cells. Whilst, they are recognized as promising biomarkers for diseases diagnosis, prognosis and therapy, their purification, selective enrichment, and characterization remains immersely chanllenging. The overall objective of the current project is to develop an optofluidic plarform able to manipulate and characterize single EVs and facilitate their efficient purification and quantitation from biological sample. This platform can be applied to a wide range of biological matrices and addresses the most challenging technological bottleneck in EVs research.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Extracellular vesicles (EVs) describe distinct populations of small (30-200 nm) and large (500 nm-2 μm)microvesicles actively or passively secreted by cells. Whilst, they are recognized as promising biomarkers fordiseases diagnosis, prognosis and therapy, their purification, selective enrichment, and characterization remainsimmensely challenging. The goal of the current proposal is to develop an optofluidic platform able tomanipulate and characterize single EVs and facilitate their efficient purification and quantitation frombiological samples. The optofluidic platform will incorporate a dynamically reconfigurable optical lattice(constructed from a liquid gradient refractive index - L-GRIN microlens) for separation followed by a viscoelasticfocusing module for single EV imaging. The strength of the interaction between a nanoparticle and the opticallattice will depend on the optical polarizability of the particle, thus providing a size selection criterion to allowidentification and isolation. Put simply, we aim to develop a size-selective optofluidic platform integrated fornon-invasive EVs sorting and fractionation that can be applied to a wide range of biological matrices andaddresses the most challenging technological bottleneck in EVs research.

Оригинален текст от CORDIS (на английски).

Участници

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH · ZuerichКоординаторШвейцария