TRANSLIGHT · Light-dependent composition of transcriptional complexes in plants
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2019-09-16 → 2021-09-15
- Финансиране от ЕС
- 160 932 €
- Участници
- 1
- Схема
- MSCA-IF
Линиите свързват координатора с партньорите.
Накратко на български
Светлината и температурата влияят върху работата на ензима РНК полимераза II, който контролира активирането на гените при растенията. Разбирането на този процес помага да се разбере адаптацията на растенията и може да подобри добива от посевите.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Light-dependent composition of transcriptional complexes in plants
Due to their sessile nature, plants have evolved very sophisticated mechanisms to respond to changes in environmental conditions. Light and temperature are among the most important environmental inputs, which trigger changes in the expression on hundreds of genes. The signalling pathways that transduce the light and temperature signals in plants have been investigated for many years and several components, and their mechanism of action, are known. However, whether these inputs directly impinge on the transcriptional machinery to modulate gene expression is not known. This project aims to answer this question by studying the effect of light on the activity and accumulation of the RNA polymerase II (RNAPII), and by identifying proteins involved in different moments of the transcription cycle during the response to light. This is an important question that will allow researchers to gain knowledge on how plants adapt to different light regimes, which has a huge impact on plant biology. Moreover, findings from this project could be employed for biotechnological uses in order to improve crop performance.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Due to their sessile nature, plants have evolved very sophisticated mechanisms to respond and adjust to the changes in environmental conditions. They do so through interconnected signaling pathways that trigger global changes in gene expression. Information about transcriptional regulation in response to different stimuli in plants relies mostly on the identification of the signaling pathways and transcription factors involved in a particular response. Importantly, investigations in yeast and humans indicate that the activity of the RNA Polymerase II (RNAPII) complex itself is subjected to a tight regulation by multiple interacting factors that respond to endogenous and exogenous stimuli. Therefore, environment-dependent changes in the activity of this transcriptional machinery seem a plausible new layer of regulation to fine-tune the transcriptional response to external stimuli in plants. In this proposal, we aim to use two complementary approaches based on quantitative mass spectrometry to isolate protein complexes in plant cells at different phases of the transcription cycle and, more important, in response to a major environmental stimulus, light. The first approach relies on the use of deactivated CRISPR/Cas9 to target proximal promoter regions and isolate proteins complexes involved in early events of light-induced transcription. In the second approach, we will identify RNAPII-associated complexes specific of transcription initiation and late elongation/termination. It relies on the use of antibodies that recognize Ser5P and Ser2P marks in the RNAPII, hallmarks of transcription initiation and termination, respectively, to isolate the region-specific RNAPII-associated complexes. Together, these two approaches will allow us i) to identify region-specific transcription machinery components in plants, both conserved and likely plant-specific ii) to study the dynamic composition of these complexes during the response to light.
Оригинален текст от CORDIS (на английски).
Участници
- AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS · MadridКоординаторИспания
Връзки
Данни: CORDIS, © Европейски съюз