SafeFood · Tracing allergenic plants in food products
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2019-08-05 → 2021-08-29
- Финансиране от ЕС
- 187 572 €
- Участници
- 1
- Схема
- MSCA-IF-EF-SE
Линиите свързват координатора с партньорите.
Накратко на български
Нови методи за секвениране на ДНК се тестват за бързо откриване на бактерии или алергени в храни и лекарства. Това помага за повишаване на безопасността на продуктите чрез намаляване на разходите и времето за анализ в лабораторията.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Tracing allergenic plants in food products
SafeFood aimed to develop a protocol to implement the cutting-edge Nanopore sequencing technology for rapid, on-site identification of contaminants such as allergenic plant ingredients and bacteria in complex, multi-ingredient food or pharmaceutical products. Enriching the mixture of DNA has always been the most challenging aspect of generating data for molecular biology. PCR and target sequencing are examples of enrichment. For the first time in sequencing history, enrichment can be done bioinformatically. Bioinformatical enrichment reduces costs and the time spend in the lab. However, little is known on the performance, applications, and limitations of such methods. During this action, a protocol for the identification of contaminants using third generation sequencing and selective sequencing was developed to contribute to safer food and pharmaceutical industries.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The presence of undeclared allergens in food products poses a serious health risk to consumers and results in important economic losses for the food industry. The detection of allergens is typically performed using immunological methods. DNA-based methods complement immunoassays and provide higher specificity and sensitivity, especially in processed foods. DNA-based identification of allergens can currently only be performed using well equipped laboratories which can be difficult to implement in an industry settings where the identification should be fast and on-site. In SafeFood, I will develop a protocol to implement cutting-edge Nanopore sequencing technology for rapid, portable identification of allergenic plant ingredients in complex, multi-ingredient food products.DNA-based identification of allergenic plant species has been challenging due to the lack of variation of the traditional DNA barcodes. I will implement more robust species identification methods based on whole plastid genomes. First, I will compile a comprehensive open-access database of allergen species’ plastomes. Then, DNA extraction and library preparation will be optimized for an on-site protocol tailored to the constraints of the food industry. I will create artificial mixtures of complex food, with varying concentrations of allergens to test the method’s sensitivity, specificity and repeatability and compare the results to those of immunoassays. This innovative solution will solve an industry challenge by allowing on-site, fast and accurate allergen identification. By being hosted at a leading DNA service company, BaseClear, and with the collaboration of a specialised academic partner in EU food safety analyses, RIKILT, I will apply my expertise in plant genomics, develop skills in food chain analyses, in R&D and entrepreneurship. At the completion of this fellowship, I will be able to apply this knowledge and skill to further address societal challenges in the food industry.
Оригинален текст от CORDIS (на английски).
Участници
- BASECLEAR BV · LeidenКоординаторНидерландия
Връзки
Данни: CORDIS, © Европейски съюз