H2020Индивидуална стипендия2020–2022

DIFTERIA · Development of a simple and rapid assay for early diphtheria diagnosis.

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2020-03-01 → 2022-02-28
Финансиране от ЕС: 174 806 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Дифтерията се изследва чрез създаването на бърз тест, който открива токсина на бактерията само за шест часа. Това помага за по-точно диагностициране на болните и своевременно лечение, за да се ограничи разпространението на заразата.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Development of a simple and rapid assay for early diphtheria diagnosis.

Before the introduction of vaccine, diphtheria was a leading cause of childhood death around the world. With the evidenced rise in cases, it seems that diphtheria is a re-emerging old foe of humankind and could become a potential major global threat. Diphtheria is relatively easy to prevent, with vaccinations given to babies. Yet approximately 15% of the world’s infants do not have access to it. In a few low- and middle-income countries, the disease is still causing sporadic infections or outbreaks in unvaccinated and partially vaccinated communities. Increasing global travel is contributing to the spread of diphtheria to areas where it has not been seen for many years. Toxigenic Corynebacterium diphtheriae and C. ulcerans, the diphtheria agents, pose a serious risk for people with low level of immune protection, the number of which has increased in Europe due to low adult re-vaccination rate, vaccine hesitancy and COVID-19 pandemic’s backsliding on childhood vaccinations. Effective laboratory diagnostics makes it possible to indicate patients with diphtheria and carriers, thereby preventing the pathogen from capturing new territories. The current diphtheria diagnostic methodology is complex and time-consuming. Considering this, scientists of the EU-funded DIFTERIA project have raised the issue of a reliable diagnostic method to prepare for possible outbreaks. The Lateral Flow Immunoassay (LFIA) was developed, which detects the diphtheria toxin (DT) in just six hours of a liquid bacterial culture incubation. Implementation of this novel test will improve the accuracy of the diphtheria diagnosis in Europe and worldwide, enabling the timely initiation of treatment and preventing the spread of the pathogen in the community.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

During 1990s, there was an epidemic of diphtheria in Russia and other countries of the former USSR affecting about 200, 000 people with 5000 deaths. Although diphtheria incidence in Russia has declined substantially, recent reports from several EU countries indicate circulation of toxigenic C. diphtheriae among migrants from Asia and Africa as well as children’s deaths among indigenous population. Toxigenic C. diphtheriae poses a serious risk for unvaccinated persons and persons with low level of immunological protection, the number of which in recent times in Europe has increased. The International system of quality control revealed poor state of laboratory diagnosis of diphtheria in EU countries, which means we are not prepared for the possible outbreaks. This is mostly due to the absence of simple, standard and reliable diagnostic methods. The leading element in the diphtheria laboratory diagnostic approach is a detection of the bacterial toxin. In this regard, it is proposed to develop non-apparatus immunochromatographic strip (ICS) test for diphtheria toxin (DT) indication. In addition a liquid selective medium for the clinical sample cultivation will be designed. A combination of the ICS test and the medium will represent a novel assay for early diphtheria diagnosis. The reagent kit will be commercially produced. The availability of simple, reliable and affordable ICS assay for DT detection will increase the accuracy of diphtheria diagnosis in EU and other countries and reduce the duration of its execution up to 6 hours (instead of 5-7 days as recommended by the WHO manual). The efficient early diphtheria diagnosis will enable timely initiation of anti-diphtheritic treatment that will save lives, prevent the development of complications (heart failure, paralysis), as well as stop the spread of the disease through initiation of preventive measures (vaccination and antibiotic prophylaxis) in case of disease outbreaks.

Оригинален текст от CORDIS (на английски).

Участници

SENOVA GESELLSCHAFT FUR BIOWISSENSCHAFT UND TECHNICK MBH · WeimarКоординаторГермания