H2020Индивидуална стипендия2020–2022

THyM · Why is Transparent Hypocotyl Mutant showed reduced phototropic response?

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2020-03-01 → 2022-02-28
Финансиране от ЕС: 203 149 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Ролята на конкретен протеин-транспортер се изследва чрез наблюдение на растения с прозрачни стъбла, които не се огъват правилно към светлината. Разбирането на този механизъм помага за оптимизиране на растежа на младите растения и повишаване на добивите от културите.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Why is Transparent Hypocotyl Mutant showed reduced phototropic response?

Plants capture sunlight efficiently by bending towards favorable light conditions by a mechanism known as phototropism. The phototropic response is ubiquitously conserved across the plant kingdom, contributing to the optimal seedling establishment and higher yields in crop plants. The directional light leads to a light gradient across the photosensory tissue, such as coleoptiles in monocots or hypocotyls in dicot plants. Blue light photoreceptors-phototropin detect the light gradient to activate downstream signaling, establishing auxin gradient leading to asymmetric growth of the photo-stimulated stem. Despite progress in understanding the molecular mechanisms of phototropism, the connecting link between light perception and auxin redistribution remain unclear. Thus, the Fankhauser group conducted a genetic screen for altered phototropism mutants and identified a gene encoding an ABC (ATP-binding cassette) transporter as essential for phototropism. The project's primary objective is to identify the function of AtABC in phototropism. The Atabc mutant is characterized by a transparent hypocotyl which allowed us to test the importance of the light gradient across photo-stimulated hypocotyl for phototropic bending. Our results indicate that the Atabc mutant is specifically defective for hypocotyl phototropism as gravitopic hypocotyl reorientation remains normal in the mutant. Normal early light signaling events in the Atabc mutant and the requirement of active photoreceptor phot1 to explain the aberrant growth re-orientation in the Atabc mutant suggest that AtABC functions upstream of light perception. The study of optical properties of etiolated seedlings and shallow light gradient experiments allowed us to conclude that the light gradient in the photosensory hypocotyl is altered in the Atabc mutant.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Plants can reorient their growth towards a favorable light environment to optimize photosynthesis in a process called phototropism. This process is initiated by the phototropin blue light receptors, perceiving light gradients to trigger downstream signaling leading to the asymmetric distribution of auxin across the photo-stimulated stem. The Fankhauser lab showed that AtABC, an ABC (ATP-binding cassette) transporter family protein is important for phototropism. AtABC shares homology with Drosophila transporters, which are involved in eye pigment precursor transport and play a vital role in insect vision. In addition to reduced phototropism, Atabc mutants have transparent hypocotyls. The primary objective of this project is to understand the function of AtABC in phototropism and to test whether this transporter is required for light gradient establishment across the hypocotyl. First, I will characterize the Atabc mutant at the tissue, cellular and subcellular levels (e.g. staining for cell wall components) to determine what defect underlies the transparent hypocotyl phenotype. Using several approaches including confocal microscopy to visualize light-activated proteins and fiber-optic techniques, I will measure the light gradient across the hypocotyl of wild type and Atabc mutants. I will characterize phototropin signaling using biochemical and microscopic approaches to determine at which signaling step AtABC is required. To characterize AtABC, I will determine its expression pattern and subcellular localization using GFP-tagged AtABC. Together with the phenotypic characterization of the mutant, this will provide testable hypotheses regarding the substance(s) transported by AtABC. Finally, to determine the functional conservation of AtABC in other plants, I will characterize Brasicca rapa mutants defective in the orthologous gene. The functional characterization of AtABC may provide key insights into light gradient establishment in a plant photosensory organ.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITE DE LAUSANNE · LAUSANNEКоординаторШвейцария