Biochip-images · A Breast Cancer Biomarker Point of CarePOCDiagnostic Platform Integrating Dual-Functionalized Nanoparticle for Magnetic Gradient Ranking and Electrochemical Sensing

A Breast Cancer Biomarker Point of CarePOCDiagnostic Platform Integrating Dual-Functionalized Nanoparticle for Magnetic Gradient Ranking and Electrochemical Sensing

This MSCA titled ‘Biochip-images’ for short aimed to implement novel approaches towards the development of a healthcare diagnostic assay capable of addressing the need for improved cross functional and adaptive therapeutics design. Now more than ever, it is clear that society stands to gain significantly from such advancements. Moreover, the recent and continued worldwide COVID-19 pandemic, and associated variants, have highlighted the clinical and societal benefits associated with early diagnosis and the importance of advanced assay development. In this project, the MSCA Fellow focused on three major objectives: 1) surface functionalisation of magnetic NPs by coating with both antibodies and redox probes to develop dual-NPs for biomarker capture and electrochemical amplification, 2) understanding the mechanisms associated with integration of PDMS microfluidic channels, towards the constructions of a novel POC device for biomarker diagnostic associated with dual-NPs and 3) initial fabrication design concepts of Mag and Me arrays to develop a microchip for Mag ranking and electrochemical sensing of MP and MB. This project has advanced the development of a new POC concept by integrating knowledge of magnetic nanoparticles (MP), advanced magnetophoresis, and a sensitive microelectrode sensing. Outcomes include; 1) Development of a novel method to isolate the target MP from the free MP, by use of a magnetic susceptibility gradient array. 2) The MP developed here has been labelled with both redox and biological properties, which allows for improved amplification as well as biomarker capture. 3) As a POC concept, the device could combine both the magnetic susceptibility gradient array and electrode array, enabling simplification of the sampling procedure and improved diagnostic efficiency (however, this would require further testing to verify). 4) Several Invention Discovery Applications (IDAs) have been submitted to the University. A parallel objective of the MSCA Individual Fellowship is to foster the development and progression of the individual researcher which is also report on below. In undertaking this MSCA, it has provided the Fellow with a framework to conduct this project within the top university of University College Dublin (UCD), working with access to world-class expertise within the Centre for Bio-Nano Interactions. Here the Fellow gained significant experience and knowledge not only within the core research objectives but also in project management, scientific writing, and proposal development. Following the conclusion of MSCA, the Fellow has secured a role as a senior scientist in LumraDx based in the UK, and has been able to utilise the knowledge and skills gained during the project towards the development of COVID ultra diagnostic assays within the Research and Development Department.

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