H2020Индивидуална стипендия2019–2022

InScope · Integrated analysis of regulatory networks modulating seed coat permeability in natural accessions

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2019-11-01 → 2022-01-01
Финансиране от ЕС: 196 708 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Пропускливостта на обвивката на семената при растението Arabidopsis се анализира чрез изследване на гена ARR16 и влиянието му върху метаболитите. Това помага за разбирането на процеси като покълването и издръжливостта на семената при стрес.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Integrated analysis of regulatory networks modulating seed coat permeability in natural accessions

Seed coat permeability affects key traits that impact seed quality, such as dormancy, longevity, and germination tolerance to abiotic stress. Seed coats are formed of several overlying cell layers that accumulate large amounts of metabolites such as polyphenols, lignin, lignan, polysaccharides, cutin and suberin, whose properties impart physical and chemical resistance to the cells. A number of mutants related to seed coat properties have been isolated and they often have altered seed coat permeability. The regulatory mechanisms that modulate seed coat permeability are, however, not well understood. As a preliminary study for this project, a method for quantitative analysis of seed coat permeability was established and used in a genome-wide association study (GWAS) with Arabidopsis accessions. Type-A ARABIDOPSIS RESPONSE REGULATOR 16 (ARR16) was identified as a novel causal gene of seed permeability that encodes a component of the signal transduction pathway for the plant hormone cytokinins (CKs). The overall objective of this project was to elucidate the molecular mechanisms underlying seed coat permeability variation between naturally occurring Arabidopsis accessions. In particular, this project aimed to address three key questions: (i) what is the causal cis-sequence controlling ARR16 expression and its effect on seed coat permeability? (ii) which seed coat metabolites and/or structures are regulated by ARR16? and (iii) how is the transcriptional network of the CKs signaling pathway structured during seed coat development? The project consisted of three experimental, and one management, work packages (WPs).

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The European seed market is in constant expansion and improving seed quality is a key factor towards meeting the increase in demand. The seed coat is constituted from multi-cell layers that surround the embryo that will become the future plant. Seed coat properties and composition have a significant impact on seed quality. Notably, mutant seeds with permeable seed coats have defects in germination phenotypes such as lower dormancy, shorter longevity and reduced stress tolerance. The regulatory mechanisms that modulate seed coat permeability are, however, not well understood. The candidate has performed a genome-wide association study in Arabidopsis thaliana and identified ARR16 as a novel regulatory gene affecting permeability, this Arabidopsis thaliana response regulator (ARR) is a signaling component for the plant hormone, cytokinins (CKs).The project objective is to determine the transcriptional network involving ARR16 that controls permeability in natural accessions. In work package 1 (WP1), the spatio-temporal expression of ARR16 will be examined in seed tissues of permeable and non-permeable accessions with GFP reporter lines. Also, we will generate mutants with CRISPR/Cas9 gene edited haplotype copies, aiming to precisely define the cis-elements in the ARR16 promoter that determine seed permeability. Next, cytological analyses will be carried out on seed coats of ARR16 mutants to ascertain the composition and structural basis of the modified permeability (WP2). Finally, transcriptomes of mutant and wild-type seed coat cells isolated by laser microdissection will be analyzed, aiming to reveal CKs signaling networks mediated by ARR16 in seeds (WP3). The proposed project is feasible and appropriate for a Marie Skłodowska-Curie Individual Fellowship since the expected outcomes are innovative and generate knowledge for both fundamental and industrial research, while the candidate will improve his skills and knowledge of a specialized research area.

Оригинален текст от CORDIS (на английски).

Участници

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT · ParisКоординаторФранция