STOCHELEC · Stochastic electrochemistry for catalysis and analysis of carboneous bio-electrodes.

Stochastic electrochemistry for catalysis and analysis of carboneous bio-electrodes.

The STOCHELEC project (MSCA-IF agreement #844746), proposes an innovative solution to preserve the activity of biological catalysts at electrified interfaces, introducing the development of a 4th generation of bioelectrochemical sensors. In classic electrochemical biosensors, enzymes immobilized on an electrode as a self- assembled monolayer (SAM) are exposed to a strong and static electrical field disturbing their active 3D structure and thus often preventing free enzymes’ dynamics that leads to a loss of enzyme activity. In order to increase the lifetime of these biologically structured interfaces, one must adjust the polarization profile to the actual dynamics of the system being used. The development of a 4th generation biosensors is necessary to benefit from the high activity and natural selectivity of biological entities combined with electronic transducers. Reaching these goals will allow monitoring precisely the concentration of specific biomarkers and triggering the redox reaction of important molecules and macro-molecules, controlling their activity in-vivo. These molecular architectures are of tremendous importance for the development of next generation biotechnological instruments including health sensors for point of care and implantable devices, and for energy conversion systems such as biofuel cells. This project aimed to acquire more knowledge on long-term operational stability of bio-electrochemical systems. The long-term goal is to dynamically stimulate the electrochemical interface to preserve and to increase the operational stability and activity of those systems. The first objective is to establish a biocompatible electrified interface using renewable materials. We successfully synthesized and used fluorescent carbon nanoparticles whose biocompatible properties are establish, thus providing a promising basis for the development of implantable devices. The second objective was to study the influence of alternating electrical polarization waveform frequencies on the bio-electrochemical reaction yields, and thus to interrogate the validity of continuous polarization in bio-electrochemical sciences. The final goal of the proposal proposed a novel electroanalytical technic based on the stochastic stimulation of the electrical interface, mimicking thermal stochastic fluctuations.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз