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КартаВръзкиТемиБългарияНощта (K-TRIO)Мария Склодовска-КюриЗа ученициЗа учителиМетодологияИзненадай ме
H2020Обмен на изследователи2020–2024

NoBiasFluors · Non-biased fluorescent dyes as markers of drugs for optical in cellulo and in vivo imaging

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период
2020-01-01 → 2024-12-31
Финансиране от ЕС
671 600 €
Участници
7
Схема
MSCA-RISE

Линиите свързват координатора с партньорите. За проекти отпреди 2014 г. CORDIS не винаги дава точни координати. Тези точки са на ниво град или държава.

Накратко на български

Червените флуоресцентни багрила се разработват така, че да не се трупат изкуствено в определени части на клетката, например в митохондриите. Това помага да се разбере по-точно как се разпределят в живия организъм нискомолекулните лекарства.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Non-biased fluorescent dyes as markers of drugs for optical in cellulo and in vivo imaging

Fluorescence imaging of biological processes in live animals is an excellent non-invasive bioanalytical tool. Due to the intrinsic properties of biological tissues, only red-fluorescing dyes can be used for the imaging. Currently available dyes of this type exhibit important disadvantages including their clear bias towards particular intracellular organelles, for example mitochondria. Therefore, distribution of drug~dye conjugates in live animals is often dominated by the dye, rather than by the drug. The problem is especially prominent if the drug is a low-molecular weight compound. The majority of currently used in clinics drugs are compounds of such type. NoBiasFluors worked on solving this problem by developing non-biased red-fluorescing dyes suitable for in vivo imaging of distribution of low molecular weight drugs. NoBiasFluors findings will allow substantial expanding our knowledge on the mode of action of typical drugs.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Imaging of distribution of drugs in mice delivers accurate information for confirmation that the mechanism of action elaborated in cell-based assays is also operative in vivo. These data are critical for the transfer of drug discovery process from pre-clinical to clinical phase. To enable the imaging, drugs should be labeled with easily detectable moieties, e.g. radioactive markers and fluorescent dyes. Ideally, the markers should not affect in vivo properties of the drugs that can be better achieved with radioactive markers, since they can be selected to be small: e.g. a single atom marker 18F applied in positron emission tomography. Despite this intrinsic advantage, PET suffers from safety issues, since radioactivity is harmful to humans and environment. In terms of safety optical imaging is much better and, therefore, in future can replace PET. However, fluorescent dyes compatible with the optical imaging are usually extended pi-systems carrying overall positive or negative charge. Their conjugation strongly affects properties of the majority of medium sized and low molecular weight drugs that limits the applicability of this method in drug discovery. The interdisciplinary and intersectoral consortium NoBiasFluors consisting of 4 academic and 2 industrial teams aims at achieving a breakthrough solution of this problem. We will develop non-biased red and near infrared fluorescent dyes, which are compatible with in vivo optical imaging and do not affect properties of drugs upon their conjugation. This goal will be achieved by the careful optimization of dye structure, polarity and charge. We will confirm the functionality of the developed dyes for labeling of representative drugs (anticancer N-alkylaminoferrocene-based prodrugs, D-peptides targeting Alzheimer’s disease) and binders of biomolecules (nucleopeptides and lectins) and monitoring their distribution both in cellulo and in vivo (for a selected labeled drug).

Оригинален текст от CORDIS (на английски).

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Данни: CORDIS, © Европейски съюз