H2020Индивидуална стипендия2020–2022

NanoFUNC · Functionalisation of ultra-bright all organic nanoparticles for super resolution imaging in intact brain preparations

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2020-01-01 → 2022-04-23
Финансиране от ЕС: 196 708 €
Участници: 1
Схема: MSCA-IF-EF-RI

Линиите свързват координатора с партньорите.

Накратко на български

Органични наночастици се разработват за високоразрешаващо изображение на биомолекули в тъкани на мозъка. Те помагат за разбирането на молекулните механизми при здравето и болестите чрез наблюдение на процесите в естествената им среда.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Functionalisation of ultra-bright all organic nanoparticles for super resolution imaging in intact brain preparations

Being able to observe the dynamics of biomolecules in their native biological environment is key to understanding the molecular mechanisms at play in health and disease. Fluorescence imaging is a major technology to allow such observation, which has provided a wealth of information in various fields including cancer research and neuroscience since its earliest developments. This technology requires bright, stable, small, spectrally relevant, targetable, and non-toxic fluorescence emitters. Nanoparticles (NPs) are powerful and versatile platforms developed by chemists and physicists to enable safe and efficient bioimaging, but bringing all these features together into a single emitter has not been achieved to date. The objective of the NanoFUNC project was thus to develop the next generation of NPs displaying all the above properties into a single NP. In the past 2 years, we have succeeded in synthesising, characterising and using in biological tissues ultra-bright, size tuneable (down to 10 nm, i.e. the size of endogenous proteins such as antibodies), red-emitting (i.e. in the biological transparency window), all-organic nanoparticles as well as explored strategies for functionalisation to render them targetable to cellular targets of interest.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Nanotechnologies have the potential to revolutionise our understanding of devastating diseases such as cancer or neuropathologies. By combining light-emitting nanoparticles (NPs) and super-resolution imaging, it is possible to observe living tissues at the molecular scale, resulting in images of unprecedented detail. To this aim, the ideal NP should be bright, stable, small, spectrally relevant, versatile, targetable, and non-toxic. These features have been sought after by chemists, physicists and biologists alike for more than a decade but their presence in a single NP has not been achieved to date. The objective of the NanoFUNC project is thus to develop the next generation of NPs displaying all the above properties.We propose to achieve this by conferring targeting properties to the latest all organic NPs of the host laboratory already displaying promising spectral characteristics. We will first validate the functionalisation of these new tools on model cellular systems before applying them to track neurotransmitter receptors deep in intact brain slices of healthy and schizophrenia rat models. We expect this project to impact all communities benefiting from advances in bioimaging including neuroscience, medicine and cell biology as a whole. We thus envisage to patent the obtained NPs for use in marketable bioimaging applications.The transfer of knowledge between the expertise of the host in nanochemistry and that of the experienced researcher in neurobiology provides a unique opportunity to successfully complete this highly interdisciplinary project. Moreover, the mobility of the researcher back to France in an internationally renowned laboratory is sure to enhance her career prospects as she aims to become an independent researcher in the near future. Together, the host and the researcher will strive for excellent scientific results and build a career plan involving training, management and communication to bring the researcher to full professional maturity.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITE DE BORDEAUX · BordeauxКоординаторФранция