HALOSPATH · Halogen metabolism of brown macroalgae and its biological significance in the context of pathologies - An omics approach

Halogen metabolism of brown macroalgae and its biological significance in the context of pathologies - An omics approach

Macroalgal pathogens are a threat to the sustainable development of macroalgal mariculture and natural populations in marine ecosystems, causing disease outbreaks and significant losses. The lack of proper posteriori containment and disease management measures has increased the urgency to study macroalgal pathologies. The nature and epidemiology of macroalgal pathogens is dramatically understudied, limited to their phylotaxonomy. Recent studies have provided some genomic and proteomic information in brown macroalgae but nothing is known about the nature of metabolites, involved in macroalgal host-pathogen interactions. HALOSPATH intended to elucidate the dynamic changes in the metabolic profiles in defense mechanisms of two brown macroalgae Ectocarpus and Laminaria against pathologies, focussing on identifying the roles of any halometabolites involved in the process, using an integrated approach of time-series comparative metabolomics, and RNA-seq transcriptomics. The metabolomic datasets were generated for Ectocarpus siliculosus infected with oomycetes Eurychasma dicksonii 4018/1 and 4018/4, identifying 21 differentially expressed metabolites in the infection. Lipidomic analysis identified 419 lipid metabolites of which 231 lipids were significantly involved in lipid remodelling during host-pathogen interaction in Ectocarpus-Eurychasma pathosystem showing the upregulation of fatty acyls and sphingolipids concomitant with the downregulation of phospholipids with increasing infection. The metabolomic analysis of Laminaria digitata from two different geographic locations co-cultivated with Laminariocolax tomentosoides displayed distinct DEM profiles. 115 genes were differentially expressed in L. digitata co-cultivated with L. tomentosoides mostly involved in photosynthesis, ROS metabolism, cell wall and post-translational modification and autophagy-induced defense responses. Thus, HALOSPATH provided in-depth insights into the metabolic and transcriptomic changes occurring during the infective success of these pathogens in Ectoacrpus and Laminaria that could be exploited for better understanding of host-pathogen interaction in seaweeds.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз