H2020Индивидуална стипендия2020–2022

MingleIFT · Multi-color and single-molecule fluorescence imaging of intraflagellar transport in the phasmid chemosensory cilia of C. Elegans

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2020-03-01 → 2022-06-15
Финансиране от ЕС: 175 572 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Транспортът на протеини в сензорните влакна (цилии) на червея C. elegans се наблюдава с помощта на специална микроскопия. Това помага да се разбере как се сглобяват компонентите на тези структури и как се променят те при външни стимули или мутации.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Multi-color and single-molecule fluorescence imaging of intraflagellar transport in the phasmid chemosensory cilia of C. Elegans

The overarching objective of this project was to develop a multi-color and single-molecule imaging/image analysis toolkit to understand (i) how different ciliary components assemble into anterograde IFT trains to enter the sensory cilia of C. elegans and (ii) how this process is perturbed during chemotaxis. To achieve the overall objective, the research methodology was structured into 3 aims, which are as follows: Aim 1: Develop a multi-color and single-molecule imaging toolbox in chemosensory cilia of C. elegans. Aim 2: Unravel how different ciliary proteins reach the base of the cilia, assemble into anterograde IFT trains and enter the cilia. Aim 3: Determine how IFT dynamics and cilia structure is perturbed by mutations of ciliary proteins as well as in response to external stimuli. The progress made during this project is briefly highlighted below and elaborated in greater detail in the next section. Aim1: A simple imaging strategy, termed small-window illumination microscopy (SWIM), was developed in order to perform long duration, fast, dual-color, single molecule imaging in C. elegans. Further, a tracking and image analysis pipeline was developed to study protein dynamics in sensory neurons as well as map the ultrastructure of sensory cilia. Aim2 and Aim3: There are a several studies that have emerged from performing SWIM microscopy in the sensory cilia of C. elegans.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Sensory cilia are essential ‘antenna-like’ organelles that protrude out of many eukaryotic cells, acting as signal transducers, enabling cells to sense and respond to the external environment. The model system for this proposed study, chemosensory cilia of C. elegans are well characterised and enable the animal to sense water soluble effectors in the environment for chemotaxis. Cilia consist of an axoneme encapsulated with a signalling protein-rich ciliary membrane. The axoneme, which is a microtubule-based core structure, acts as a template for a specialised intra-cellular transport, intraflagellar transport (IFT). IFT trains are large protein complexes that mediate contacts between motor proteins (IFT kinesins and IFT dynein) and ciliary cargoes, crucial for the formation and maintenance of the cilia, with anterograde IFT trains moving outwards from the ciliary base to deliver ciliary building blocks to the ciliary tip and retrograde IFT trains moving from the ciliary tip to the ciliary base to recycle the waste products. The overarching objective of this project is to grasp the connection between chemosensory function of cilia (initiating chemotaxis), IFT and ciliary length-regulation using single-molecule imaging techniques. In order to achieve this, (i) I will develop a multi-color and single-molecule imaging toolbox to study IFT in the phasmid chemosensory cilia of C. elegans. (ii) Using the toolbox, I will obtain a mechanistic understanding of turnaround dynamics of the IFT machinery (IFT motors and components of the IFT trains), during normal IFT. (iii) A comprehensive understanding of normal IFT will enable discovery of the subtle adjustments made by the IFT machinery, and its effect on the cilia length, in response to chemical cues in the external environment. Ultimately, the goal is to understand how organism level tactic response is interlinked with intracellular transport in the ciliary antennas of individual cells, using C. elegans as a model system.

Оригинален текст от CORDIS (на английски).

Участници

STICHTING VU · AmsterdamКоординаторНидерландия