H2020Индивидуална стипендия2020–2022

MicroMix · Microbial Conlonization in Confined Chaotic Mixtures

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2020-05-01 → 2022-08-06
Финансиране от ЕС: 196 708 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Бактериалните биофилми в почвата се изследват чрез микрофлуидни устройства, които имитират движението на течностите и действието на антибиотиците. Това помага да се разбере как смесването на веществата влияе върху растежа на бактериите в трудно наблюдаеми среди.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Microbial Conlonization in Confined Chaotic Mixtures

The project tackles the issue of biofilm development in the porous microstructure of the soils, where it is submitted to the porous media flows and the subsequent mixing. Bacteria strive in these hidden and complex environments. Most times, their can be observed attached to the surface, upon which they have formed biofilms. Since soils are generally opaque, it is impossible to monitor in the environments the formation of such biofilms. The project aims at reproducing the mixing conditions of the soil, and observe biofilm development in it. First, we needed to build the tool, which we successfully achieved thanks to microfluidics devices which mimics the soil's mixing properties. Second, we observed the growth of biofilm in various mixing conditions (i) without antibiotics and in homogeneous conditions and (ii) in the presence of a mixture of antibiotics, for which the mixing properties define the viable space available for the growth of the biofilm. Results: 1) We managed to recreate the transport properties of a 3D soil in a quasi-2D microfluidic device, by unveiling the strong ties between the dispersion and the chaotic mixing properties. 2) We observed that the biofilm would grow wider in the geometries which would disperse AND mix solute better. 3) In the presence of antibiotics, we saw that the biofilm would grow better in the less mixing geometries, where the viable space would be larger.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Subsurface bacteria represent a fundamental, yet poorly known, component of the Earth’s biosphere. These communities are key in biogeochemical cycles and in a range of problems in Environmental and Geosciences, ranging from water resources management and bioremediation, to CO2 sequestration and geothermal energy. Until recently, the opacity of 3D porous media-the natural habitat of subsurface bacteria-had prevented in situ and in vivo imaging of bacterial dynamics in such environments. Recent experimental and theoretical breakthroughs at the host institution have led to the discovery that flows in natural porous media are chaotic in nature. Since chaotic mixing is known to yield and sustain strong chemical gradients at micro-scale, this discovery challenges the assumption of homogeneous nutrient distributions, broadly-used in current models of subsurface microbial processes. The goal of MicroMix is thus to explore the effect of chaotic mixing on bacterial growth and colonization in 3D porous media under positive stimuli (WP1: mixing-limited nutrient resources) and negative stimuli (WP2: antibiotic source, nutrient rerouting by bioclogging). To do so, we will develop a novel bioreactor system, primarily based upon coupling high-resolution Laser Induced Fluorescence and optical index matching, which will allow us to obtain the first joint imaging of chemical landscapes and bacterial colonies in 3D porous media. The project builds upon the combined expertise of the ER in the field of biomicrofluidics, of the supervisor in mixing dynamics, and of the secondment supervisor on biofilm dynamics in porous media. Through a detailed career development plan, a tailored training program and access to key experimental facilities and scientific networks, MicroMix will ensure an efficient re-integration of the ER and place him at the forefront of research on environmental fluid dynamics.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITE DE RENNES I · RENNES CEDEXКоординаторФранция