H2020Индивидуална стипендия2021–2023

OMIT · The role of microbial Oxylipins in the MIcrobe-hosT dialogue

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-11-01 → 2023-10-31
Финансиране от ЕС: 196 591 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Микробните оксилипини са мазнини, произвеждани от бактериите в червата ни, които действат като сигнали. Разбирането на тези вещества помага да се разбере как микробите влияят върху здравето и развитието на различни заболявания.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

The role of microbial Oxylipins in the MIcrobe-hosT dialogue

Background & Significance: In the last decade the gut microbiome has become recognised as a co-evolved super-organ, intimately linked to our own physiology. Comprising trillions of microbes, including bacteria, of different phyla, our gut microbiome performs a range of key functions from aiding in digestion to immune system education. In addition, we now know that certain gut bacteria synthesis membrane associated glycine and sphingolipids which display signalling functions. Problem: However, our knowledge of the types of lipid signals produced by our gut bacteria is limited in particular our knowledge of a subset of lipids known as oxylipins. Oxylipins are generated from the oxidation of unsaturated fatty acids. Human oxylipins (hOXY), are well characterised and are key regulators of inflammation. However, the diversity, functions, and impact of microbially derived oxylipins (mOXY) are not well understood. For instance, what are the types and ranges of mOXY that are produced by residents of our gut microbiome? Are they dietary dependant? What are their roles? How are they differentially influencing health and disease and how can they be exploited for health benefits? OMIT aims to address some of the key questions related to mOXY following 5 specific research objectives (RO) listed below: 1. RO1: develop and validate an ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method for the comprehensive extraction and analysis of oxylipins from faeces and bacterial supernatants. 2. RO2: perform bacterial co-incubations (i.e., bacteria + precursor) to determine the oxylipins produced by specific bacteria. 3. RO3: determine the function of mOXY in microbe-microbe interactions. 4. RO4: determine the range and type of mOXY in faeces from healthy and diseased individuals. 5. RO5: determine the function of mOXY in microbe-host interactions. Why is this important for society? OMIT represents a highly original and innovative approach to significantly improve our understanding of mOXY and their potential importance towards our health / disease status. This will open avenues for microbiome-based approaches to disease risk monitoring and management.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

OMIT aims to examine the role of microbial Oxylipins in the MIcrobiome:hosT dialogue. Oxylipins liberated from the oxidation of fatty acids are appreciated as key chemical signals perceived by both microbe and man. Human oxylipins (hOXY), comprising eicosanoids, docosanoids and octadecanoids are well characterised; they are implicit in many biochemical and signalling pathways in humans, including cell migration, proliferation, chemotaxis, immune reactions and most importantly, they are key regulators of inflammation. However, the diversity, functions, impact and mechanistic roles of microbially derived oxylipins (mOXY) are not well understood. State-of-the-art ultra-performance liquid chromatography-mass spectrometry (MS) platforms (secondment at Waters MS Headquarters, UK) will be developed and validated for oxylipin analysis in faecal samples and bacterial supernatants derived from co-incubation experiments (i.e. bacteria and oxlipin precursor). The latter will facilitate the discovery of novel mOXY. Additional bacterial biofilm assays will be performed to examine the role of mOXY in microbe-microbe interactions. To determine the contribution of mOXY in health and disease, biobanked samples from healthy volunteers and Crohns disease patients will be analysed. In vitro cell culture experiments and gene expression profiling will determine the function of mOXY in microbe-host interactions. During this project, the applicant will receive high quality training through research that will significantly improve scientific knowledge and provide a strong platform for building an independent research career. In line with the Work Programme for the MSCA fellowships, the knowledge and competencies gained by the applicant will add to developing her potential to achieve professional maturity and leadership in the field so that she may continue to make significant contributions to society and the economy.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK · CorkКоординаторИрландия