H2020Индивидуална стипендия2021–2023

EPmIC · Controlling the susceptibility of biological cells to pulsed electric field treatment by using ion channel modulators

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-03-01 → 2023-11-05
Финансиране от ЕС: 155 289 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Йонните канали в клетките и техните модификатори се изследват, за да се разбере как те влияят върху реакцията на клетката към импулсни електрически полета. Това помага за развитието на методи за лечение на рак, сърдечна аблация и доставка на гени.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Controlling the susceptibility of biological cells to pulsed electric field treatment by using ion channel modulators

The project addressed the issue of understanding the effects of ion channels and ion channel modulators on the response of biological cells to pulsed electric field treatment. This is important, as it contributes to the advancement of knowledge in the field of electroporation, which has implications for various medical applications including cancer treatment, cardiac ablation, and gene delivery. The scientific objectives of the project were to identify ion channels contributing to changes in transmembrane voltage following electroporation of cells in vitro, develop computational models describing the cells’ electrophysiological response to electroporation, and explore the potential use of ion channel modulators to influence cell susceptibility to electroporation. The training objectives were linked to acquiring new transferable skills, new experimental/technical skills, and extending the modeling skills of the researcher.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The integrity of the cell membrane, while essential for life of any biological cell, presents a barrier that sometimes needs to be transiently disrupted in order to deliver therapeutic molecules inside the cell. High-intensity pulsed electric fields (PEFs) are used increasingly in medicine to achieve such increase in cell membrane permeability via a phenomenon called cell membrane electroporation. PEF-based clinical applications include gene therapy techniques, DNA vaccination, electrochemotherapy, non-thermal tumor ablation, and cardiac ablation. Depending on the desired outcome of the PEF treatment, the targeted cells must either survive or die. However, different cell types exhibit different susceptibility to PEF treatment, with some cells being killed at lower pulse amplitude than others, which often presents a disadvantage that limits the safety and efficiency of PEF treatment. In this action I aim to design an approach that will allow us to increase or decrease cell’s susceptibility to PEF treatment in a controlled, clinically applicable way. The approach is based on using modulators of membrane ion channels, that can influence the extent and longevity of post-pulse membrane depolarization – a hallmark of membrane electroporation. My idea is on the one hand inspired by increasing amount of evidence showing the involvement of ion channels in PEF-induced cell response, and on the other hand by the fact that ion channel modulators are already successfully used in treatment of various diseases and we can expect exciting development of new modulators in the near future. The design will be guided by state-of-the-art microscopic techniques and computational models, including molecular dynamics simulations, particle-based simulations, and finite element modeling, that will help elucidate the molecular mechanisms of membrane depolarization and choose the appropriate modulators and pulse parameters for fine-tuning the treatment outcome.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERZA V LJUBLJANI · LjubljanaКоординаторСловения