STARSS · Super Time-resolved Fluorescence Anisotropy with Switchable States
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2020-04-01 → 2022-03-31
- Финансиране от ЕС
- 191 852 €
- Участници
- 1
- Схема
- MSCA-IF-EF-ST
Линиите свързват координатора с партньорите.
Накратко на български
Взаимодействията между протеините и промените в тяхната форма в живи клетки се изследват чрез нов метод за измерване на тяхното въртене. Това помага за по-доброто разбиране на клетъчната биология и процесите при откриването на нови лекарства.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Super Time-resolved Fluorescence Anisotropy with Switchable States
Viable experimental techniques able to reveal and quantify protein-protein interactions and protein conformational changes can have a significant impact on cell biology and drug discovery. However, with state-of-the-art techniques, it is very challenging to precisely, efficiently, and specifically follow these processes directly in a physiologically relevant system. An effective tool is the use of fluorescence anisotropy techniques that studies rotational diffusion properties of biological systems, providing direct insight into biological processes such as molecular binding, enzymatic activity, and protein assembly. The novel approach developed during the project is called selective time-resolved fluorescence anisotropy with reversible with switchable states (STARSS), and it aims to measure protein complex formation in living cells overcoming the limits imposed by the photo-physics of fluorescence. STARSS takes inspiration from super-resolution resolution techniques in fluorescence microscopy, and it translates these powerful concepts into improved angular resolution and temporal range in rotational studies. The main objectives of the project were to develop a prototype optical system able to perform the experiments, screen for the best labeling solution for the samples, and develop proof-of-concept applications to showcase the potential of the technique.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Viable experimental techniques able to reveal and quantify protein-protein interactions and protein conformational changes can have a significant impact on cell biology and drug discovery. Fluorescence anisotropy (FA) has been widely employed in biomedical research as a tool for high-throughput screening applications, to study the binding of small molecules to protein and characterize protein-protein interaction. Despite the enormous potential of the FA technique, the major limiting factor is the inability of probing the system past the fluorescence lifetime, which in the most favorable cases lasts for a few nanoseconds, setting an upper limit to the time scales that can be addressed with the technique, which translates in an upper limit of few nanometers of molecular size.Reversibly switchable fluorescent transitions have the potential to revolutionize the capability of FA tools for the study of large molecular aggregates, overcoming the limits imposed by the finite fluorescence lifetime, and providing a practical and highly sensible way of measuring rotational diffusion processes with a practically unlimited upper bound on molecular sizes. This proposal aims to develop a novel fluorescent anisotropy technique, named Super Time-resolved Anisotropy with Reversibly Switchable States (STARSS), designed to measure rotational mobility all-across the time scale from nano- to micro-seconds, which will enable to discern clusters from free rotating molecules in situ and with high angular precision. The coupling of STARSS observables and microscopy will provide a powerful tool to reveal the dynamics of protein complexes inside the compartments of living cells, shedding new light on a multitude of biological processes.
Оригинален текст от CORDIS (на английски).
Участници
- KUNGLIGA TEKNISKA HOEGSKOLAN · StockholmКоординаторШвеция
Връзки
Данни: CORDIS, © Европейски съюз