H2020Индивидуална стипендия2021–2023

CCMuPWA · Characterize corpus callosum-mediated local and global inhibitory effects with novel MRI-compatible photonic crystal fiber-based multifunction probe and wireless amplified NMR detector in rat brain

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-01-01 → 2023-06-30
Финансиране от ЕС: 205 353 €
Участници: 2
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Мозъчното телце и неговото влияние върху невронните връзки се изследват чрез нови сензори и ЯМР при плъхове. Това помага за по-доброто разбиране на механизмите при заболявания като епилепсия, аутизъм и шизофрения.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Characterize corpus callosum-mediated local and global inhibitory effects with novel MRI-compatible photonic crystal fiber-based multifunction probe and wireless amplified NMR detector in rat brain

The structural anomalies of corpus callosum (CC) in patients are found highly correlated with a wide range of disorders, e.g., epilepsy, autism, schizophrenia, and mental retardation. However, it remains unclear about the causal contributions of CC-mediated functional changes to these disorders and exactly how the changes influence the local cortical circuitry. The project’s overarching goal was to overcome the challenges to optimize the multi-modal fMRI platform and characterizing the brain activity upon optogenetic callosal activation with higher spatial resolution using cutting-edge technologies. Therefore, during the MSCA, we first successfully combined fMRI with fiber optic mediated calcium recordings and optogenetics, i.e., multi-modal fMRI, to study the balance of excitation/inhibition in the barrel cortex in rats by pairing optogenetic corpus callosum activation with ascending thalamocortical activation. Second, we designed and fabricated a novel microstructured optical fiber-based probe integrated with calcium recording, optogenetic manipulation, and fluid injection function. Third, we modified WAND to be incorporated into the multi-modal fMRI platform to achieve brain dynamic signals with enhanced sensitivity from the barrel cortex. Last, we developed a novel bilateral line scanning method combined with the MOF-based probe to better decipher CC-mediated interhemispheric inhibition with layer-specificity. This proposal merged the neuronal dynamic signals to the functional mapping, partially solved the challenges for CC study across multiple scales in the brain, and enable novel applications of the multi-modal fMRI platform to better decipher the brain function/dysfunction in normal and diseased animal models. For instance, we investigated the role the entorhinal cortex plays in Alzheimer’s disease.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The structural anomalies of corpus callosum (CC) in patients are found highly-correlated with a wide range of disorders, e.g., epilepsy, autism, schizophrenia and mental retardation. However, it remains unclear about the causal contributions of CC-mediated functional changes to these disorders and exactly how the changes influence the local cortical circuitry. Lately, we have successfully combined fMRI with fiber optic mediated calcium recordings and optogenetics, i.e., multi-modal fMRI, to study the balance of excitation/inhibition in the barrel cortex in rats by pairing optogenetic corpus callosum activation with ascending thalamocortical activation. However, it remains challenging to maintain high sensitivity to the brain dynamic signal and better decipher CC-mediated unique cellular (neuron/astrocyte) or layer-specific contributions to the local cortical or global whole-brain fMRI signals. Therefore, the goal of this proposal is to optimize the multi-modal fMRI platform and to characterize the brain activity upon optogenetic callosal activation with higher spatial/temporal resolution using two cutting edge technologies, wireless amplified nuclear MR detector (WAND) and photonic crystal fiber (PCF). Previously, we have implanted a wireless RF coil into the rat body to achieve a high signal-to-noise ratio and spatial resolution for in vivo kidney imaging. The modified WAND will be incorporated into the multi-modal fMRI platform to achieve brain dynamic signal with enhanced sensitivity from the barrel cortex. Next, we will merge it with a novel PCF-based probe integrated calcium recording, optogenetic manipulation and fluid injection function. This proposal will merge the neuronal and astrocytic dynamic signals to the functional mapping, solve the challenges for CC study at multiple scales in the brain, enable novel applications of the multi-modal fMRI platform to better decipher the neuroglial interactions in normal and diseased animal models for future studies.

Оригинален текст от CORDIS (на английски).

Участници

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV · MUNCHENКоординаторГермания
MICHIGAN STATE UNIVERSITY · East Lansing, MichiganСъединени щати