H2020Индивидуална стипендия2021–2023

MANTARGET · Super resolution imaging of nanoPMOs for cancer drug delivery

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-01-04 → 2023-01-03
Финансиране от ЕС: 184 708 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Наночастици от органичен силицид (nanoPMOs) се проследяват с висока разделителна способност, за да се види как влизат в клетките на рака на простатата. Това помага за разработването на по-селективна терапия с по-малко странични ефекти от стандартните методи.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Super resolution imaging of nanoPMOs for cancer drug delivery

Prostate cancer is one of the leading causes of deaths in Europe and US and is becoming a dramatically increasing malignancy in Asian countries. An important increase in the detection of tumors of small size has also been observed, estimated to at least 450,000 new cases of prostate cancer in 2018 in Europe. Prostatectomy, radiotherapy, or hormono therapy are currently the standard treatments of prostate cancer. However, all these therapies are very invasive and often not effective. Nanotechnology can offer new opportunities for overcoming the drawbacks of conventional therapy providing innovative solutions for a selective and side-effect free therapy for prostate cancer. Therefore the development of novel nanosystems and their biological evaluation is a topic of great importance in the European strategy for improving health. This is an ambitious challenge that requires a multidisciplinary approach (ranging from synthetic chemistry to biology) and a strong technical expertise. In this project, we proposed to combine the state-of-the-art technologies towards the treatment of prostate cancer: nanoPMOs (Periodic Mesoporous Organosilica Nanoparticles) and super resolution microscopy. NanoPMOs are a recent family of nanoparticles (NP) with promising properties for drug delivery. The full potential of this novel platform has still to be explored. In order to study and evaluate the behaviour of nanoPMOs in cells, we proposed to use an innovative microscopy technique: super resolution Stochastic Optical Reconstruction Microscopy (STORM). STORM allows for imaging with 20 nm resolution and therefore was ideal for tracking nanoPMOs. Thanks to this the dynamic of internalization of the nanoPMOs in prostate cancer cells through the newly identified over-expressed mannose-6-phosphate (M6P) receptor, and their degradability behaviour in cells was investigated. This project allowed a better understanding of the nanoPMOs-prostate cancer cell interactions through a potent imaging technique and provided the necessary information for the further application of nanoPMOs in the field of nanomedicine for cancer theranostics.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

In recent years, nanomaterial-based various drug delivery carriers have been developed for the potential targeted cancer therapy. However, still the advanced study for optimization of size, shape, surface chemistry of the nanocarrier and observation of biological interaction and evaluation of such nanocarrier are not well-developed that limit their use for the efficient clinical applications. Therefore, superior understandings of the cellular interaction of the nanocarrier with different physicochemical properties are essential and challenging for improved the cancer treatments. In this project, we will focus on the optimization of different physicochemical properties of nanocarrier such as size, shape, surface chemistry and etc via investigation of its biological interaction and evaluation by a potent imaging technique. For this purpose, we will synthesize a library of efficient biodegradable drug delivery carrier periodic mesoporous organosilica nanoparticles (nanoPMOs) withcontrolled size and shape and then functionalized with different targeting ligands with the controlled number per nanoparticle for mannose-6-phosphate receptor over-expressed prostate cancer. Then we will study the nanoPMOs-prostate cancer cell interactions, internalization pathway and intracellular degradation of nanoPMOs through the stochastic optical reconstruction microscopy (STORM). We will further evaluate the drug delivery efficacy of nanoPMOs and toxicity mechanism of drug loaded nanoPMOs to support microscopic observation. Thus the result of this biological interaction and evaluation of nanoPMOs with different physicochemical properties via super-resolution microscope STORM will help to develop a novel drug delivery carrier for prostate cancer therapy with optimal properties for clinical applications.

Оригинален текст от CORDIS (на английски).

Участници

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS · ParisКоординаторФранция