H2020Индивидуална стипендия2020–2022

SupraRNA · A Comprehensive Supramolecular Approach for an RNA vaccine for Influenza A (H1N1)

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2020-05-01 → 2022-06-30
Финансиране от ЕС: 212 934 €
Участници: 1
Схема: MSCA-IF-EF-ST

Линиите свързват координатора с партньорите.

Накратко на български

Супрамолекулярни наночастици се тестват като средство за безопасно доставяне на РНК ваксина срещу грип А (H1N1) в организма. Това е важно, защото позволява по-бързо и евтино производство на ваксини, които да се адаптират към бързо променящите се мутации на вируса.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

A Comprehensive Supramolecular Approach for an RNA vaccine for Influenza A (H1N1)

Influenza is a contagious respiratory illness caused by influenza viruses. These viruses have several types (namely A, B and C), which are rapidly changing, and in severe cases can result in hospitalization or death. In India, since 2009, the influenza A H1N1 variant has caused many deaths each year and is an ongoing health concern. Vaccines are available against influenza, but are expensive and their slow production means they cannot be produced fast enough to protect against each mutation of the disease. A new type of vaccine based on nucleic acids, DNA and RNA, show promise for overcoming this problem, as they can be more rapidly produced to prevent or treat pathogens that are rapidly evolving, and have lower production costs. However, the bare DNA is prone to degradation in the body, which will limit its effect and application in the clinic. To overcome this challenge Dr. Sikder has been developed supramolecularly assembled nanoparticles for the safe delivery of nucleic acid vaccine with ultimate target to make silence H1N1 virus gene. Further the knowledge gained has paved the way for DNA/RNA vaccines against other diseases to be developed in the future. Overall, this proposal aims to lay the groundwork for extending the scope of nucleic acid vaccines by exploring the potential of supramolecular assemblies as a delivery vector. A series of π-amphiphiles have been synthesized to prepare supramolecularly assembled nucleic acid delivery system for the vaccination of influenza A H1N1 strain. The central π-amphiphile moiety has been functionalized with the nucleic acid strand via a redox responsive disulfide bond, and hydrophilic oligo-oxy aryl groups connected via a hydrogen bonding unit to promote self-assembly. Steric stabilization has been afforded to the surface decorated nucleic acid nanoparticles to protect from enzymatic hydrolysis in the complex biological environments, through co-assembly with an analogue π-amphiphile which has been alternatively decorated with poly(ethylene glycol) (PEG) chains. The therapeutic efficacy of the nano-assembly has been evaluated through transfection efficacy in macrophage cell lines.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

*The development of vaccines is key for disease prevention, and is a major focus globally in the healthcare sector. Seasonal influenza is an acute respiratory infection caused by influenza viruses which circulate in all parts of the world. Seasonally, it remains a persistent health threat and has been declared an epidemic in some states. Typical vaccines have been less effective against rapidly evolving pathogens such as influenza. A new class of vaccines based on nucleic acids, namely RNA, have recently been developed and show immense promise due to their robust nature, short manufacturing times and enhanced efficacy. Here we propose an RNA delivery system based on a supramolecular assembly approach for the vaccination of influenza A H1N1 strain. Specifically, a π-amphiphile will be used as the platform molecule for covalent RNA conjugation and delivery. A messenger RNA (mRNA) targeting the hemagglutinin (HA) gene from a model influenza virus strain will be employed as the therapeutic (H1N1/PR8-HA). The central π-amphiphile moiety will befunctionalized with the mRNA strand via a redox responsive disulfide bond, and hydrophilic oligo-oxy aryl groups connected via a hydrogen bonding unit to promote self-assembly. Steric stabilization will be afforded to the surface decorated mRNA to protect from enzymatic hydrolysis in the complex biological environments, through co-assembly with an analogue π-amphiphile which has been alternatively decorated with poly(ethylene glycol) (PEG) chains. Efficient intracellular transport of the delivery vehicle to achieveoptimum mRNA transfection will be achieved through the incorporation of a TAT-peptide on the PEG chain end. The therapeutic efficacy of the nanoassembly will be evaluated through transfection efficacy in macrophage cell lines. Overall, this proposal aims to lay the groundwork for extending the scope of RNA vaccines by exploring the potential of supramolecular assemblies as a delivery vector.

Оригинален текст от CORDIS (на английски).

Участници

THE UNIVERSITY OF BIRMINGHAM · BirminghamКоординаторОбединеното кралство