H2020Индивидуална стипендия2020–2022

HiPhy · High Phytosterol variants towards improved feedstocks and biofortification of crops

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2020-09-01 → 2022-08-31
Финансиране от ЕС: 196 708 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Фитостеролите в растения като тютюна и арабидопсиса се изучават, за да се открият механизмите за тяхното създаване и натрупване. Това помага за подобряване на културите и по-ефективно производство на суровини за фармацевтичната и козметичната индустрия.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

High Phytosterol variants towards improved feedstocks and biofortification of crops

Phytosterols are valuable biomolecules used as nutrients and as feedstock material for the pharmaceutical and cosmetic industries. The innovative and fundamental challenge biologists and breeders are faced with is to develop cell technology engineering programs and valuable crops dedicated to the large scale production of phytosterols, which are currently extracted from agricultural waste materials. The goal of the proposal is to identify master regulators of phytosterol biosynthesis and accumulation in genetic models. In fact, phytosterols have essential cellular functions in plant growth and development, acting as key membrane components and membrane reinforcers, as signals in development, and as precursors of brassinosteroids, a class of growth regulators. As such, the concentration of phytosterols and the equilibrium between free sterols and conjugated sterols is highly regulated in order to comply with cellular homeostasis. The goal of the HiPhy project was to identify key players of phytosterol biosynthesis and accumulation in the genetic models thale cress (Arabidopsis thaliana) and tobacco (Nicotiana tabacum).

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Phytosterols (PS) are valuable biomolecules used as nutrients and as feedstock material for the pharmaceutical and cosmetic industries. The challenge biologists and breeders are faced with is to develop bioengineering programs and valuable crops dedicated to the large-scale production of PS, which are currently extracted from agricultural waste materials. The goal of the proposal is to identify master regulators of PS biosynthesis and accumulation in plants. In fact, PS have essential cellular functions in plant growth and development, acting as key membrane components and membrane reinforcers, as signals in development and as precursors of brassinosteroids, a class of growth regulators. As such, the concentration of PS is highly regulated in order to comply with cellular homeostasis. To reach the goal, we will carry out a functional molecular characterization of a tobacco variant carrying a yet unknown semi-dominant mutation responsible for PS overproduction and Arabidopsis thaliana mutants exhibiting a suppressor phenotype of a defect in the production of precursors of isoprenoid lipids, such as PS. The work plan is to apply advanced genomics for next-generation mapping, leading to the elucidation of mutations in genes of interest (GOI) that regulate PS accumulation. The validation of candidate GOI will be done by genome editing and the role of novel alleles in PS metabolism will be performed by various functional and metabolomic studies. Finally, the combined expression of selected GOI with steroidogenic enzymes will be assessed by implementing in folia expression assays in Nicotiana benthamiana.

Оригинален текст от CORDIS (на английски).

Участници

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