L6b · Target specific responses of layer 6b (L6b) neurons in the mouse medial prefrontal cortex (mPFC) upon fear memory retrieval
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2020-11-02 → 2022-11-01
- Финансиране от ЕС
- 224 934 €
- Участници
- 1
- Схема
- MSCA-IF
Линиите свързват координатора с партньорите.
Накратко на български
Неврони от специфичен слой (L6b) в префронталния кортекс на мишки се анализират при възникване на спомени за страх. Това помага за по-доброто разбиране на механизмите при тревожност, посттравматично стресово разстройство и шизофрения.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Target specific responses of layer 6b (L6b) neurons in the mouse medial prefrontal cortex (mPFC) upon fear memory retrieval
One of the fundamental challenges in neuroscience research is understanding how diverse sensory inputs lead to specific behavioural outcomes and the basic networks or neuron types that elicit such behaviours. Here, in the current project, as an overall objective, we tried to address as profoundly as possible the role of one single neuron type, L6b of the infralimbic area of the medial prefrontal cortex (IL-mPFC), in the context of fear and anxiety disorders. L5 and L6 neurons constitute a significant source of output neurons mPFC. The project's outcome will provide more insights into the critical role of mPFC in top-down executive control of attention, fear memories and post-traumatic stress disorder (PTSD), and neurological disorders such as schizophrenia.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
One of the fundamental challenges in neuroscience research is to understand how diverse sensory inputs leading to the specific behavioral outcome and what are the networks underlie eliciting such behaviors. This question may only be answered when measurements of structural and functional properties of individual neurons are integrated into a coherent framework, which would then allow numerically simulate (in silico) different networks to predict possible behavioral outcomes. The ‘building block’ for a network model relies on anatomical and physiological data such as neuron input, neuron output, and physiological properties collected from animal experiments. The input can be determined by reconstructing dendrites, functional properties of the neuron can be estimated by obtaining electrophysiological measurements, and output can be determined by reconstructing by their 3D axon branching pattern. Current proposed project work will address some of the building blocks such as projection targets of single neurons upon a specific behavioral expression and their presynaptic partners that are responsible for eliciting such behaviors. Specifically, I will investigate the electrophysiological properties and anatomical connectivity of L6 neurons (L6b) of the medial prefrontal cortex (mPFC) using state-of-the-art techniques and tools such as modified rabies virus labeling and neuron tracing, DREADD technology and optogenetics in a transgenic mouse line that selectively expresses Cre-recombinase in a subpopulation of layer 6b neurons. Exploring mPFC neural circuits may not only provide more in-depth insight on to its involvement in cognitive behaviors but also in a variety of psychiatric conditions such as post-traumatic stress disorders (PTSD). The outcome of this study will establish the functional role of L6b of the mouse medial prefrontal cortex to its specific involvement in eliciting specific behavioral outcomes.
Оригинален текст от CORDIS (на английски).
Участници
- THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD · OxfordКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз