CHLARABIDOX · Discovering genome-wide thiol-dependent metabolic regulation in photosynthesis with redox chemoproteomics
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2020-06-01 → 2023-12-08
- Финансиране от ЕС
- 263 732 €
- Участници
- 2
- Схема
- MSCA-IF
Линиите свързват координатора с партньорите.
Накратко на български
Промените в окислителното състояние на протеините при водорасли и растения показват как светлината влияе върху фотосинтезата. Тези знания помагат за създаването на биогорива и подобряване на адаптацията на посевните култури към промените в климата.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Discovering genome-wide thiol-dependent metabolic regulation in photosynthesis with redox chemoproteomics
Photosynthesis is a biological process that converts sunlight (renewable energy) and carbon dioxide (an important greenhouse gas) into oxygen (used to breathe) and organic matter (nutrients). Thus, photosynthesis not only is an essential process for life on Earth, due to increasing concern on global warming caused by atmospheric CO2 accumulation, it is also a process that allows tools for reducing atmospheric CO2, which is one of the Sustainable Development Goals (13. Climate Action). In this regard, research interest on photosynthetic organisms is gaining even more attention with the aim of providing tools for facing the challenge of global warming. In our project we have used two photosynthetic model organisms: Chlamydomonas reinhardtii, a single-cell alga, and Arabidopsis thaliana, a land plant. We aim to study modifications of proteins that change their conformations and activities in response to internal or external stimuli, such as light, that have an impact on the operation of the photosynthetic apparatus. Our focus is a type of modification that changes the oxidation state of the protein. The findings of our research might have applications for the field of plant science: i) in the case of algae, by developing new biotechnological strategies to manipulate their metabolism for production of biofuels and bioproducts, and ii) in the case of plants, by generating new useful tools to develop innovative strategies of crop acclimation to future climate conditions, given that the climatic change is expected to have an enormous impact on agronomic yields worldwide.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
Most organisms exhibit a diurnal metabolic cycle, especially phototrophs, whose metabolism is strictly dependent on light. Dark-light transitions are accompanied by dramatic changes in the redox state of photosynthetic components, which drives redox-based post-translational modification of protein cysteines, whose oxidation state can considerably impact protein activity, and thus regulate metabolism. Given the central role of redox metabolism in biology, the operation of thiol-disulphide based switches are well-appreciated as a metabolic acclimation strategy, and the study of cysteine modifications in proteomes is a major interest of contemporary biology. The objective of CHLARABIDOX is to go beyond inventories of redox modified proteins by monitoring the proteome-wide dynamics of disulphide-dithiol status in the context of a diurnal metabolic cycle in phototrophic eukaryotes, specifically, the green alga Chlamydomonas reinhardtii and the land plant Arabidopsis thaliana. An innovative chemoproteomic isoTOP-ABPP approach will be used in an experimental design with deep temporal resolution to capture a good fraction of the proteome with site specificity and quantitative information about reactivity. The discoveries will be made in the context of a body of literature on thioredoxin-dependent redox regulation of central carbon metabolism, which will serve as a priori validation. The outcome of the project is a proteome-wide view of the operation of regulatory redox sensors, anchored to accompanying rich datasets on physiology, metabolic potential, transcriptomics, proteomics and central metabolites, which would inform the operation of light-driven metabolic networks. Both systems are compatible with downstream modelling of diurnal metabolic fluxes and validation by reverse genetics approaches. A long term impact on strategies for manipulating metabolism for biofuels production, or manipulating photosynthesis for better acclimation to climate change is also envisioned.
Оригинален текст от CORDIS (на английски).
Участници
- UNIVERSIDAD DE SEVILLA · SevillaКоординаторИспания
- THE REGENTS OF THE UNIVERSITY OF CALIFORNIA · OaklandСъединени щати
Връзки
Данни: CORDIS, © Европейски съюз