H2020Индивидуална стипендия2022–2024

INDICANTS · INnovative DIagnostiCs for BANana paThogens Surveillance

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2022-01-01 → 2024-12-31
Финансиране от ЕС: 219 051 €
Участници: 2
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Диагностичните инструменти за бързо откриване на болести по бананите, като например Fusarium wilt, се разработват за употреба директно на място. Те помагат за навременното идентифициране на патогени, което е важно за продоволствената сигурност и икономиката на много държави.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

INnovative DIagnostiCs for BANana paThogens Surveillance

Boosted by the globalization of economy, emerging and invasive plant pests and pathogens are a major threat to the sustainability of agricultural industries and food security. Timely surveillance of pathogens associated with their accurate identification and sensitive detection from host are key for prevention or informed management of plant disease outbreaks. Banana is world's most important fruit in terms of production, with over 114 million tonnes produced globally each year. International banana trade remains a major economic pillar for many countries, providing income and sustenance to millions of people. Nevertheless, diseases of banana are becoming increasingly important globally, resulting in reduced yields and escalated disease management costs. The most important vascular diseases include Fusarium wilt disease, caused by Fusarium oxysporum f. sp. cubense, particularly the TR4 strains (FocTR4). Banana Xanthomonas wilt (BXW) caused by Xanthomonas vasicola pv. musacearum (Xvm), Moko disease (MOKO) and banana blood disease (BDB) caused by Rasltonia solanacearum and Ralstonia syzygii subsp. celebesensis, respectively. Efficient surveillance and disease management require the availability of Point-of-Care (POC) diagnostics that can be operated on-site. The objectives of the project were to develop some POC diagnostic tools like LAMP (loop-mediated isothermal amplification) for these diseases, to validate them through inter-laboratory tests and missions in the field and to collaborate with a private company to produce some ready-to-use kits. All these scientific objectives have been successfully achieved, along with those related to training and the two-way transfer of knowledge between the researcher and the host.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Bananas are major staple in developing countries and the most eaten fruit in Europe. Global banana production is constrained by several diseases that are responsible for yield losses and low productivity of bananas and severely compromise food security. Particularly banana production is seriously threatened by four vascular diseases, Fusarium wilt disease of banana caused by the fungus Fusarium oxysporum f.sp. cubense (Foc), Moko and banana blood diseases, caused by Ralstonia solanacearum and R. syzygii subsp. celebesensis, respectively) and Xanthomonas wilt of banana caused by Xanthomonas vasicola pv. musacearum. Efficiency of surveillance and plant disease management requires the availability of Point of care (POC) diagnostics that can be operated directly on site. INDICANTS project focuses on (i) the development of a low-cost multi-pathogens LAMP (Loop-mediated isothermal amplification) detection kit, and its validation via an interlaboratory test and field surveys. (ii) the evaluation of an emergent technology (CRISPR/Cas12a) for the diagnosis of Foc. (iii) the test of an innovative microneedle patch for DNA extraction from plant. This project relies on a two-way transfer of knowledge and expertise between the applicant and Stellenbosch University: the applicant will access to areas where the diseases are present, will acquire through hands-on training and training-through-research, new expertise and skills in genetics/biotechnology, thereby strengthening the future career prospects. The partner organisation will gain experience and knowledge on bacterial banana diseases and LAMP method. Actions of protection and exploitation of the outputs will be set up, with the involvement of an industrial partner. Besides the scientific publications and conferences, the results will be disseminated to different audiences: the general public (website, press and TV), plant protection services (workshops) MSc students (lectures), schoolchildren (Platform visits).

Оригинален текст от CORDIS (на английски).

Участници

CENTRE DE COOPERATION INTERNATIONALE EN RECHERCHE AGRONOMIQUE POUR LEDEVELOPPEMENT - C.I.R.A.D. EPIC · ParisКоординаторФранция
STELLENBOSCH UNIVERSITY · StellenboschЮжна Африка