H2020Индивидуална стипендия2020–2023

NUCLAGY · NUCLEAR DYNAMICS OF AUTOPHAGY IN PLANT IMMUNITY

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2020-11-01 → 2023-04-25
Финансиране от ЕС: 203 852 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Ядрената аутофагия при растенията изследва как протеините от семейството ATG8 разграждат компоненти в ядрото на клетката по време на вирусна инфекция. Разбирането на тези процеси помага за разработването на нови стратегии за подобряване на устойчивостта на културните растения към болести.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

NUCLEAR DYNAMICS OF AUTOPHAGY IN PLANT IMMUNITY

The NUCLAGY project aimed at advancing our understanding of how autophagy functions in plant immunity with the long-term goal to identify new strategies for the improvement of disease resistance in crop species. In general, autophagy is a well-studied degradation and recycling pathway in eukaryotic organisms, yet most of the knowledge in plants has accumulated on its cytoplasmic targets and mechanisms during development and stress responses. NUCLAGY therefore intended to particularly address the largely unexplored nuclear events of autophagy and to uncover the potential role of autophagic targets and activities in the nucleus during plant virus infection. Based on our previous observation that several isoforms of the ATG8 protein family have the capacity to localise to distinct subcompartments in the nucleus, it was hypothesised that ATG8 proteins (i) contribute to the autophagic degradation of nuclear substrates (nucleophagy), (ii) are involved in the nuclear regulation of pro- or antiviral autophagy activities and/or (iii) intersect with viral effector proteins. The following research objectives were addressed by the different working packages: 1. Identification of nuclear components associated with autophagy at normal and virus-induced conditions 2. Investigation of the functional relationship of isolated nuclear components with autophagy regulation or selective degradation 3. Analysis of the interplay of viruses and their effectors with the nuclear events of autophagy. Overall, the outcome of NUCLAGY provided strong evidence for important roles of nuclear autophagy during plant virus infection and indicated that ATG8-related functions in the nucleus can contribute both to antiviral defences and viral pathogenicity. The identification of ATG8-associated proteins revealed several novel candidates as nuclear autophagic substrates or potential transcriptional/epigenetic regulators, and pointed to a distinct nuclear subcompartment as an emerging battlefield for the autophagy-pathogen interplay. These fundamental new insights hold promise for translational applications and may serve in the future breeding-based approaches to enhance crop fitness and productivity for sustainable agriculture.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Autophagy is a major degradation and renewal system that controls cellular homeostasis and stress adaptation in eukaryotes. Emerging evidence indicates that autophagy is an integral part of the plant’s defence against pathogens and thus, a promising target to improve disease resistance in crops. Research on plant autophagy has primarily focused on cytoplasmic targets and mechanisms, yet the degradation of nuclear components (nucleophagy) and the epigenetic regulation of autophagy have recently received growing attention in yeast and mammalian systems. Whether nuclear events are of similar importance for plant autophagy is unknown and therefore, the major question to be addressed in this project. The proposed research builds on my key finding that several isoforms of the Arabidopsis core autophagy protein 8 (ATG8) localize to distinct nuclear compartments. I aim to identify the nuclear ATG8 interactomes at normal and virus-induced conditions by affinity-immunoprecipitation and mass spectrometry analysis. Candidate interactors will be functionally analysed for their potential role as transcriptional/epigenetic regulators, cargo receptors, and nucleophagic substrates in response to Cauliflower mosaic virus and Cabbage leaf curl virus. I will also investigate how these pathogens intersect with nuclear functions to manipulate cytoplasmic events of autophagy during infection. The proposed project will be carried out at the Swedish University of Agricultural Sciences under supervision of Prof. Daniel Hofius, a leading scientist and expert in plant autophagy and immunity. Hence, the project provides an excellent opportunity to expand my knowledge and experience to autophagy, while the host lab will benefit from my strong expertise in plant virology and epigenetics. Together, this synergistic effort will reveal fundamental insights into the nuclear dynamics of autophagy in plant immunity, and uncover novel autophagic mechanisms for crop improvement.

Оригинален текст от CORDIS (на английски).

Участници

SVERIGES LANTBRUKSUNIVERSITET · UppsalaКоординаторШвеция