H2020Индивидуална стипендия2020–2023

IMPED · INTEGRATED MICROFLUIDIC PLATFORM FOR PROTEOMIC AND GENETIC EXOSOME DETECTION

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2020-05-01 → 2023-07-19
Финансиране от ЕС: 225 381 €
Участници: 2
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Екзозомите са малки частици, които клетките използват за комуникация и пренасят протеини и генетичен материал. Анализът им чрез кръвни проби помага за по-лесното откриване на биомаркери, например при рак, без нужда от рискови процедури.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

INTEGRATED MICROFLUIDIC PLATFORM FOR PROTEOMIC AND GENETIC EXOSOME DETECTION

Extracellular vesicles (EVs) are tiny lipid bilayer vesicles released by cells, playing a crucial role in intercellular communication through ligand signalling and cargo transfer between cells. They transport proteins, lipids, and nucleic acids, thereby relaying critical information among cells. Exosomes, a type of EV, are particularly promising biomarkers, especially in cancer research, as they carry the biological signature of their parent cells in the form of various biomolecules. Additionally, exosomes can be easily collected through liquid biopsy, eliminating the need for invasive and risky procedures, and they can be obtained in high concentrations. However, scientists face numerous challenges as they strive to understand the roles of EVs in the body. Handling and visualizing these nanoscale particles pose difficulties, their concentration in blood is extremely low (picomoles), and they exhibit high heterogeneity in terms of size and content. Nonetheless, as researchers delve deeper into the study of EVs, they are discovering that this heterogeneity contains crucial information that could aid in identifying specific markers of interest. Consequently, one of the main challenges encountered when working with EVs as biomarkers is obtaining consistent and reliable results due to their inherent diversity. The objective of the IMPED project is to develop a comprehensive platform for studying extracellular vesicles (EVs) by integrating protein and nucleic acid detection methods. This platform aims to enable the processing and analysis of patient samples using a single device, which will encompass the extraction and purification of EVs from the sample, as well as the analysis of biomarkers present on the external membrane or encapsulated inside the vesicles.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

In order to adapt treatment to the multicity of cancer types, cancer patient management has been revolutionized in the past decades by personalized medicine. As part of this strategy, liquid biopsy has emerged as a non-invasive approach for personalized diagnostics and treatment. So far, this approach has focused on circulating tumor cells and circulating tumor DNA. However, extracellular vesicles termed exosomes, which are implicated in cellular communication and molecule transfer, have risen as promising biomarkers for earlier and more accurate detection. The IMPED project aims at developing a next-generation technology able to extract and analyse single exosomes or sub-groups of exosomes on both the proteomic and genomic level. This platform will offer beyond state-of-the-art information on the involvement of exosomes in cancer development. It has the potential to increase the precision of liquid biopsy analysis and provide clinically relevant insight for personalized cancer management. Toward this goal, the IMPED project will gather three technologies allowing low-cost extraction and analysis of exosomes from human plasma: (1) Continuous label-free extraction of exosomes from complex matrices (plasma) using thermophoresis (2) Proteomic profiling of single exosomes immobilized on a nanoarray with possibility of release (3) Droplet-based reverse transcriptase polymerase chain reaction (RT-PCR) of exosomes for RNA content analysis To our knowledge, this would be the first technology to couple intra- and extra-vesicle detection on a single/subpopulation exosome level within a single platform. The IMPED project is interdisciplinary, connecting physics, microfluidics and bioassay development for biomedical applications.

Оригинален текст от CORDIS (на английски).

Участници

INSTITUT CURIE · ParisКоординаторФранция
ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING MCGILL UNIVERSITY · MontrealКанада