H2020Индивидуална стипендия2020–2022

Stroma.Org · Functional Organization of Immunity by Lymphoid Stromal Cells

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2020-07-01 → 2022-06-30
Финансиране от ЕС: 178 320 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Лимфните тъкани и специалните зони в тях помагат на редките имунни клетки бързо да открият патогени. Разбирането на тази клетъчна архитектура може да помогне за създаването на по-ефективни ваксини.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Functional Organization of Immunity by Lymphoid Stromal Cells

The immune system faces an enormous challenge when protecting us – it must provide rapid, tailored immunity to the host without a priori knowledge of when, where and which pathogen will invade. This challenge is further amplified by the fact that the cells that should specifically recognize and fight the pathogen are extremely rare. Hence, the overarching aim of this project was to understand how such rare cells reproducibly detect the presence of pathogens in a timely manner; with the main hypothesis tested being that the lymphoid tissues where immune responses are triggered contain dedicated spatial cellular arrangements (niches) to optimize pathogen recognition and anti-pathogenic responses. Within this framework, we aimed to: 1. Identify and characterize immune cell niches within lymphoid organs that, in the steady-state, optimize immune cell homeostasis and pathogen recognition; 2. Characterize the response of the identified niches to inflammatory stimuli. By answering these questions, our data is likely to inform on the design of more efficient vaccines that consider the cellular architecture of lymphoid tissues and how they respond to the vaccination/inflammatory stimuli. Overall, the action is concluded satisfactorily. With minor deviations, its objectives and milestones were achieved resulting, thus far, in the publication 3 original research papers and 1 editorial article.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The immune system is faced with a substantial challenge – it must provide rapid, tailored immunity to the host without a priori knowledge of when, where and which pathogen will invade, and at the same avoid damage to self. Recent evidence, including my own prior work, suggests that the anatomical organization of the lymphoid organ in which the immune response is initiated determines the quality and quantity of the response. Lymph nodes (LNs) are supported by a scaffold of mesenchyme-derived stromal cells (SCs) that not only provides gross anatomical support, but also produces critical survival factors and localization/movement guidance cues to antigen-presenting cells and antigen-responsive lymphocytes during the initiation of the immune response. Despite the now emerging role of SCs in immune responsiveness, it is unclear at a molecular and cellular level how these cells support the fine-grained spatial and temporal requirements for robust immunity. Here, using novel single cell datasets, advanced highly-multiplex immunofluorescence imaging, spatial statistics, genetic targeting and in house-developed tools to track the development of immune responses, I aim to 1) create a comprehensive single cell atlas of LN organization in homeostasis, 2) unravel the rules of lymphoid SC specification and organizer function, 3) determine the impact of LN architecture on protective immunity, and 4) determine how such organization is altered in asthma, a highly prevalent chronic inflammatory airway, since alterations in SC-immune cell intercellular communication could explain increased immunity to harmless allergens, in the face of reduce immunity to viral infection and vaccination. By combining my qualifications in SC biology and LN organization with the expertise of the host lab in lung immunopathology, I have created an ambitious work plan that will serve as critical foundation to design future therapeutic interventions that benefit human health.

Оригинален текст от CORDIS (на английски).

Участници

VIB VZW · ZWIJNAARDE - GENTКоординаторБелгия