H2020Индивидуална стипендия2021–2023

ACRO · Ascidian development and cytoplasmic reorganizations: dissecting the mechanisms that pattern developmental determinants and shape embryonic development

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-03-01 → 2023-02-28
Финансиране от ЕС: 174 167 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Механизмите за пренареждане на цитоплазмата в яйцето на асцидиите определят как се разпределят веществата, които формират тялото на ембриона. Разбирането на тези процеси помага да се разбере как се определят осите на тялото при хордовите животни.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Ascidian development and cytoplasmic reorganizations: dissecting the mechanisms that pattern developmental determinants and shape embryonic development

In many animals, the asymmetries that underlie the specification of the body axes are established in the fertilized egg. For example, in frog and ascidian eggs, fertilization triggers massive cytoskeletal-dependent reorganizations of the cytosol to asymmetrically pattern developmental determinants along the prospective body axes. Thus, to understand axis specification, we must elucidate how cytoskeletal forces are organized in space and time and how eggs harness these forces to establish polarity. Despite its critical role in determining cell fate, remarkably little is known about the mechanisms that orchestrate cytoplasmic reorganization and the distribution of maternal factors in ascidians. The overall aim of this proposal was to elucidate the molecular, cellular, and biophysical processes that drive cytoplasmic reorganization and how cytoplasmic reorganization determines the localization of maternal factors that pattern the developing embryo. This project employed high-resolution microscopy, force and cell shape manipulations, and proximity biotinylation to address the following fundamental questions: 1) What are the microtubule-dependent mechanisms that regulate cytoplasmic reorganization and anterior-posterior polarity in the ascidian egg? 2) How does cytoplasmic reorganization control the precise positioning of developmental determinants? This study will shed light on the principles underlying anterior-posterior body axis specification and embryo patterning in ascidians and deepen our understanding of how embryogenesis is conserved among chordates.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

What makes a head different from a tail? In many animals, the asymmetries that underlieThe asymmetric distribution of cytoplasmic domains and RNAs is critical for asymmetric cell division and fate determination. Therefore, unraveling the mechanisms that drive the asymmetric distribution of the cytoplasm is essential for understanding animal development. Once fertilized, ascidian eggs undergo a massive cytoplasmic reorganization that precisely localizes developmental determinants (proteins and RNAs) to establish the anterior-posterior body axis. Following this reorganization, an invariant cleavage pattern predictably segregates determinants asymmetrically into blastomeres, conferring differential cell fates. Thus, polarity in the ascidian egg determines the body plan of the whole animal. Despite its vital role in establishing embryonic patterning, remarkably little is known about the mechanisms that orchestrate cytoplasmic reorganization and the distribution of developmental factors. In this project, I will use develop a multidisciplinary approach, combining high-resolution microscopy and force and cell shape manipulations, proximity biotinylation, proteomics, and transcriptomics to address the following fundamental questions: 1) What mechanisms regulate cytoplasmic reorganization and anterior-posterior polarity in the ascidian egg? 2) How is cytoplasmic reorganization translated into the precise positioning of developmental determinants? This study will shed light on the processes that define the anterior-posterior body axis and pattern of development in ascidians and deepen our understanding of how embryogenesis is conserved and has evolved among chordates. Throughout this project, I will not only acquire new technical skills but also build upon my project management, communication, and leadership skills towards my goal of becoming an independent researcher investigating how the cytoskeleton establishes cellular morphology and organization to inform cell function.

Оригинален текст от CORDIS (на английски).

Участници

INSTITUTE OF SCIENCE AND TECHNOLOGY AUSTRIA · KlosterneuburgКоординаторАвстрия