H2020Индивидуална стипендия2021–2023

RPAAL · The role of Polycomb Repressive Complex 2 (PRC2) in plant acclimation to ambient light

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-04-01 → 2023-03-31
Финансиране от ЕС: 144 981 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Епигенетичният комплекс PRC2 и неговата роля при адаптирането на растението Arabidopsis thaliana към светлината се анализират чрез проучване на растежа и фотосинтезата му. Това помага да се разбере как регулацията на гените влияе върху развитието и биомасата на растенията.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

The role of Polycomb Repressive Complex 2 (PRC2) in plant acclimation to ambient light

Sunlight is the primary source of energy for our planet. It provides the energy necessary to drive the chemical reactions involved in photosynthesis in plants. It is the key component that initiates the process and enables the conversion of light energy into chemical energy, which is stored in the form of sugars and other organic molecules. These compounds serve as the foundation for the food chain, as they are consumed by other organisms for energy and growth. Therefore, sunlight is fundamental to the sustenance of life on Earth. However, the connection of light perception and signalling with plant growth under changing environmental conditions is still not fully understood. Epigenetics refers to the study of changes in gene expression or cellular phenotype that do not involve alterations to the underlying DNA sequence. One of most well-known and extensively studied epigenetic mechanisms is histone modification. Polycomb Repressive Complex 2 (PRC2) is an evolutionarily conserved histone methyltransferase complex that catalyses trimethylation of lysine 27 of histone H3 (H3K27me3), which leads to epigenetic gene repression. In Arabidopsis thaliana, it is still not fully known how PRC2 contributes to seedling emergence and greening. In this project, we addressed the impact of PRC2 dysfunction on light signalling, photosynthesis and vegetative growth in Arabidopsis, with focus on seedling establishment and vegetative growth of adult plants. Through this project, we have shown a previously unknown connection between epigenetic gene regulation and light adaptation, which is crucial for plant vegetative growth and biomass production.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Polycomb Repressive Complex 2 (PRC2) is an evolutionarily conserved histone methyltransferase complex that catalyses trimethylation of lysine 27 of histone H3 (H3K27me3), which leads to epigenetic gene repression. In Arabidopsis thaliana (A. thaliana), CURLY LEAF (CLF) and SWINGER (SWN) are two major H3K27 methyltransferases and core components of PRC2 in the sporophyte, playing essential roles in the regulation of plant development. However, the knowledge of how PRC2 participates in operational control in plants is scarce. Recently, altered phenotypes of PRC2-depletion mutants grown under different light intensities were observed. Hence, here I raise the hypothesis that PRC2 acts in fully differentiated cells to modulate the ambient light acclimation. At first, short- and long-term light acclimation responses that require PRC2 activity will be identified, employing plant morphology and physiology tools. Next, PRC2-governed molecular mechanisms and PRC2-modulated light signaling pathway in the light acclimation will be established. Molecular tools (RNA-seq and ChIP-seq) will be used to profile transcriptome and H3K27me3 distribution to establish the patterns of epigenome and transcriptome alteration in WT and mutants. At last, the focus will expand to select other chromatin modification mutants displaying altered light acclimation responses. In summary, for the first time, dynamics of PRC2 repression in fully differentiated cells in response to light acclimation will be determined and it will lay a solid foundation for studying other chromatin modifiers in future. The project builds on combining my experience in plant physiology and ecology and gaining research experience in the field of developmental epigenetics. The fellowship proposal is designed in a way to enhance my independent thinking capacity, organizational and management skills and responsibilities, which are necessary to become an excellent and independent researcher and group leader in the future.

Оригинален текст от CORDIS (на английски).

Участници

BIOLOGICKE CENTRUM AV CR VVI · Ceske BudejoviceКоординаторЧехия