H2020Индивидуална стипендия2021–2023

MENTHOL · Metabolic heterogeneity of monoclonal bacterial cells as a biotechnological tool to produce natural compounds.

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-07-01 → 2023-06-30
Финансиране от ЕС: 160 932 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Бактерии от вида P. putida се модифицират, за да произвеждат растителни вещества като ментол и лимонен чрез разделяне на работните процеси между клетките. Този подход помага за подобряване на биотехнологичното производство на ценни индустриални съединения по устойчив начин.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Metabolic heterogeneity of monoclonal bacterial cells as a biotechnological tool to produce natural compounds.

The MSCA action MENTHOL aimed to create a new biotechnological platform for microbial production of high-value plant metabolites. The project involved the establishment of a distributed catalysis within single-species-based multifunctional bacterial populations. It was focused on producing isoprenoids such as limonene, menthol, and p-cymene, which are compounds with significant industrial relevance. Fully aligned with the sustainable development strategies promoted by the European Union, this project positively influenced microbial biotech progress. In recent decades, synthetic biology (SynBio) has advanced microbial metabolite production. For early strategies, metabolic burden of monoclonal fermentations and complex ecological interactions within synthetic consortia are yet issues to be solved. MENTHOL innovatively addressed these by decoupling complex biosynthetic pathways into minimal functional modules, to be reassembled within metabolically differentiated isogenic populations. The outcomes of this MSCA action contributed to the emergence of a novel hypothesis: programmable metabolic heterogeneity as a strategy to promote division of labour within monoclonal bacterial populations. Specific objectives of this MSCA action have been: (1) engineering a pre-programmable heterogeneous monoclonal population of P. putida cells through cutting-edge SynBio approaches (WP3); (2) engineering a P. putida strain overproducing isopentenyl pyrophosphate (IPP) (WP4); (3) model-based engineering of multifunctional P. putida populations overproducing limonene, menthol, and p-cymene (WP5-6). Additional goals were focused on fostering training and development of the researcher (WP1-2).

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

This proposal aims to change current paradigms of biotechnological production of complex plant natural compounds, specifically isoprenoids such as limonene, menthol, and p-cymene. MENTHOL main innovation lies in the synthetic decoupling of the complex biosynthetic pathways of these compounds into a distributed catalysis within monoclonal heterogeneous bacterial populations. Limonene and menthol are natural monoterpenes widely used as flavor and fragrance additives, while p-cymene is an important precursor in the bio-based production of terephthalic acid, a key precursor of Polyethylene Terephthalate (PET). Although they have been successfully produced by genetically engineered microorganisms, to reach industrial scale production there are still obstacles to overcome. On the one hand, the introduction of large biosynthetic pathways in a single bacterial strain may cause host unstable physiology and low performance; and on the other hand, the use of synthetic consortia to overcome this shortcoming can be limited by differences in the growth profile of species involved. This proposal is conceived as an alternative to avoid those obstacles, by decoupling the complex biosynthetic pathways of the target compounds into minimal functional modules, to be reassembled inside metabolically differentiated subpopulations of a single specie-based multifunctional bacterial culture. To achieve such ambitious goal, in this project we will applied cutting-edge systems and synthetic biology tools to engineer programmable metabolic heterogeneity inside a monoclonal population of Pseudomonas putida, to promote the division of labor during the production of limonene, menthol, and p-cymene using waste cooking oils as economic and reliable renewable feedstock.

Оригинален текст от CORDIS (на английски).

Участници

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS · MadridКоординаторИспания