H2020Индивидуална стипендия2021–2023

MICROBIS · Metabolites Inducers of Cross-tolerance to Biotic Stress

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-09-15 → 2023-09-14
Финансиране от ЕС: 172 932 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Метаболитните промени при растенията след климатичен стрес се изследват като възможна „памет“, която ги защитава от патогени. Разбирането на тези механизми помага за разработване на стратегии за повишаване на устойчивостта на културите и осигуряване на хранителните ресурси.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Metabolites Inducers of Cross-tolerance to Biotic Stress

The exponential increase in world population constitutes a global challenge to ensure food security. Paradoxically, crop quality and productivity are increasingly threatened by more frequent stress factors due to climate change and conversion of suboptimal soils into croplands. Compared to abiotic stressors, pathogens have wider impact on crop yield as they limit plant growth, interfere with post-harvest processes, and affect livestock and human health. Pathogens are responsible of 15% of global crop losses and compromise the equivalent of food for 600 million people. Therefore, deciphering features involved in plant defence is fundamental to complete our understanding about immunity and design biotechnological strategies to prepare plants for adverse environments. There have been important limitations that precluded our capacity to elucidate mechanisms underlying immunity. First, plant immune responses have been mostly characterised upon pathogen infection in isolation, but not in combination with abiotic stressors as often occurs in nature. Second, immune responses rely on interconnections among multiple biological components and not on individual players, as traditionally addressed. Third, defence strategies characterised in model species might differ in species of agronomical interest due to interspecific barriers. To circumvent such limitations, I investigated plant immunity using sequential designs for abiotic-biotic stressors and systemic analysis in Arabidopsis and crop species. Previous studies envisaged that metabolome reconfigurations during transient environmental changes tend to be persistent overtime. Thus, I hypothesised whether metabolite changes configured during abiotic stress conditions could act as signatures to retain “stress memory”. The overarching goal of MICROBIS was the identification of metabolic changes in Arabidopsis after exposure to abiotic stressors that enhance resilience against pathogens and set the path for translation into plants of agronomic interest. I addressed four objectives: 1. To characterise Arabidopsis immune responses under sequential stress periods reproducing natural conditions. 2. To recapitulate metabolome rewiring upon sequential stress and isolate candidate features for cross-tolerance. 3. To ascertain the biological relevance of candidate metabolites for cross-tolerance in planta. 4. To initially translate cross-tolerance mechanisms into species of agronomical interest. The outcome of MICROBIS will contribute to design strategies that efficiently reinforce immunity against frequent pathogens in plants of agronomical interest.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Ensuring food security and accessibility represents a global challenge since world population grows exponentially while crop quality and productivity is restricted by limitations on arable areas and increasing incidence of stress conditions due to climate change. Plant pathogens are major threats for optimal crop yield and preservation of the harvest. Although plant immune responses are well characterised in isolation, pathogen infections frequently occur in combination with other stress conditions. Importantly, the outcome of plant-pathogen interactions under combinatorial stress is unpredictable as the operating molecular mechanisms differ from those triggered under each individual stressor. Certain abiotic stressors ultimately confer resilience to subsequent pathogen infection, i.e. cross-tolerance, however the network of molecular events involved remains undeciphered. Recent studies suggested that metabolites could retain stress memory and modulate combinatorial stress responses, but their potential role in cross-tolerance has been barely investigated. Hence, this MSCA is aimed at identifying changing metabolites after abiotic stress periods that enhance plant tolerance to pathogens. To this end, comprehensive conditions and time-courses for sequential stress will be defined to cultivate Arabidopsis thaliana plants under frequent abiotic stressors, namely drought and temperature shifts, followed by pathogen challenge. Subsequently, metabolome changes will be profiled and systemically analysed to retrieve central features. The biological relevance of candidates will be assessed in planta. Finally, artificial intelligence algorithms will be applied to select species of agronomic interest that metabolically respond to sequential stress as Arabidopsis does and thus initiate translation of cross-tolerance strategies into crops. Consequently, this MSCA will contribute to the development of new strategies to ensure food security under current climate change conditions.

Оригинален текст от CORDIS (на английски).

Участници

CENTRE DE RECERCA EN AGRIGENOMICA CSIC-IRTA-UAB-UB · CERDANYOLA DEL VALLESКоординаторИспания