H2020Индивидуална стипендия2021–2023

PCHYPERs · PhotoChromic HYdrogen PERoxide biosensors for super-resolution imaging of hydrogen peroxide microenvironments

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-11-01 → 2023-10-31
Финансиране от ЕС: 178 320 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Водородният пероксид действа като сигнал в клетките, който се проследява чрез нови биосензори за изображения с висока разделителна способност. Те помагат да се разбере как работят процеси като заздравяването на рани и имунната реакция на нанониво.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

PhotoChromic HYdrogen PERoxide biosensors for super-resolution imaging of hydrogen peroxide microenvironments

Hydrogen peroxide (H2O2) is a cellular second messenger that is deliberately produced and is key for a healthy cellular metabolism. An adequate amount of H2O2 production and delivery is required to maintain normal cellular and tissue functions, including neural development, wound healing, and innate immune response, with an imbalance compromising such functions and potentially leading to disease. Most of the advancements on H2O2 signaling have occurred thanks to the development and use of genetically-encoded fluorescent biosensors, which connect the presence of H2O2 to a fluorescence signal that can be easily recorded on a fluorescence microscope. However, most H2O2 signaling events happen at the nanoscale, which is not possible to visualize with current biosensors, as they are not compatible with super-resolution microsopy techniques that allow for nanoscale imaging. Such lack of tools impede us to get a full understanding how these H2O2 signaling events work. The goal of this action “PhotoChromic HYdrogen PERoxide biosensors for super-resolution imaging of hydrogen peroxide microenvironments” (PCHYPERs) is to develop variants of current hydrogen peroxide biosensors that are compatible with nanoscale imaging microscopy techniques. The developed H2O2 biosensors will allow monitoring hydrogen peroxide signaling events at their natural nanoscale. The PCHYPERs action is divided into 3 objectives: Generate the nanoscale imaging compatible biosensors, known as PCHYPERs (objective 1), apply the PCHYPERs in a cellular setting (objective 2), and determine super-resolution hydrogen peroxide gradients using the PCHYPERs (objective 3). We successfully generated green fluorescent PCHYPERs (objective 1), which we were able to express in cells and obtain super-resolution images of hydrogen peroxide (objective 2). Whilst objective 3 could not be achieved in this action, due to delays in work packages related to objectives 1 and 2, this action has generated tools that allow the visualization of physiologically relevant hydrogen peroxide signaling events.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

In cells, hydrogen peroxide (H2O2) is deliberately produced and is key for a healthy cellular metabolism, and an adequate H2O2 production and delivery is required for correct cellular and tissue functions. The majority of this H2O2 production occurs in cellular microdomains, and the signalling actions in these microdomains are restricted to a very confined area. Most of these discoveries were made possible thanks to the use of genetically-encoded fluorescent biosensors that detect the changes in H2O2 concentration, including the HYPER family of biosensors. However, the details of these microdomains are missing because these biosensors rely on conventional fluorescence microscopy methods, which are limited in spatial resolution. This project aims to overcome the resolution limitation in H2O2 imaging by developing photochromic variants of the HYPER biosensors, known as PCHYPERs, that can be utilized in super-resolution microscopy. This will enable to discern the gradients of metabolic H2O2 at a high resolution, helping identify the role of H2O2 as a cellular messenger in signalling pathways and the functional implications of its disturbance.

Оригинален текст от CORDIS (на английски).

Участници

KATHOLIEKE UNIVERSITEIT LEUVEN · LeuvenКоординаторБелгия