H2020Индивидуална стипендия2022–2025

PARCA · Advance in Proteomics and Analysis of dyes and Recovery of Charred and Aged textiles

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2022-02-01 → 2025-01-31
Финансиране от ЕС: 269 003 €
Участници: 2
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Протеините и багрилата в стари вълнени тъкани се анализират чрез нов общ метод за извличане. Това помага за по-бързото разкриване на търговските пътища и технологичното ниво на миналите култури чрез изследване на обгорени археологически находки.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Advance in Proteomics and Analysis of dyes and Recovery of Charred and Aged textiles

Background. Archaeological textiles hold significant importance in comprehending past cultures and histories. They play a crucial role in confirming or validating historical sources, shedding light on the utilization of specific species, providing evidence of trade routes, and indicating technological levels. However, textiles often face complex preservation challenges, such as susceptibility to various breakdown processes like insect damage, fire, different degrees of carbonization, soil contamination, and partial or total mineralization. These textiles are typically composed of proteinaceous fibers, including those derived from hair, such as furs and wool, composed mainly of keratins (IFs) and keratins associated proteins (KAPs). In addition to the protein component, textiles are often dyed, and the identification of dyes provides valuable information for reconstructing the socio-cultural background of the textile. Aim of the project. The PARCA project addresses both components of wool textiles—dyes, specifically anthraquinone ones, and keratins and keratin-associated proteins. The aim is to individuate an innovative protocol to demonstrate the possibility of achieving extraction and characterization of both component in a single workflow, getting yields comparable to individual analytical processes. This means the sample processing time and the number of samples required is strongly reduced. The protocol is first developed on dyed and undyed wool specimens and then applied to textile mock-ups subjected to varying degrees of thermal aging, with the aim of evaluate the modifications in protein pattern step by step at different temperatures. The ultimate goal is to apply such protocols to semi- or fully charred archaeological specimens, thus providing a new tool for understanding the degradative processes linked to the events that triggered them, as in the case of the eruption of Mount Vesuvius in 79 AD, thus allowing us to look at both sides of the same coin.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

The analysis of archaeological textiles is a challenging undertaking. These precious remains have been altered to the point that discerning their composition requires the development of high specialised analytical tools, characterized by high sensitivity and great versatility. In different sites, many textiles have been excavated, some well-preserved, some with signs of carbonisation; in some cases, traces of colour are still visible. PARCA project proposes to join forces between dye analysis and proteomics to develop an innovative streamlined protocol that would combine dye and protein analysis in a single extraction to minimise sampling size while maximising the amount of information obtained. It will be achieved through the most up-to-date methodologies and instrumental developments of Liquid Chromatography-Orbitrap-Mass Spectrometry to develop protocols that will be applicable to the most degraded archaeological textiles. The LC-MS data will be correlated with FTIR and SEM analyses, to determine the threshold of protein survival by non-destructive techniques prior to proteomics. The project will be carried out for the first 2 years at the Smithsonian Institution (Washington DC-USA), in the proteomics lab under the supervision of Dr. Caroline Solazzo. The third year, incoming phase, will be carried at Sapienza University of Rome, under the supervision of Prof. Roberta Curini. At the end, archaeological relics from the Mediterranean basin will be analysed, in particular samples from Pompeii and Vesuvian areas and from Greek areas. The success of this project will result in gaining new experience in the analytical characterization of ancient textiles, by enriching my background on dyes with the acquisition of a very high level of skills in the field of proteomics and in high resolution mass spectrometry. This knowledge will allow me to reach a leading profile as independent researcher and to strongly strengthen my position in the field of Cultural Heritage Science.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITA DEGLI STUDI DI ROMA LA SAPIENZA · RomaКоординаторИталия
SMITHSONIAN INSTITUTION · Washington DcСъединени щати