H2020Индивидуална стипендия2021–2023

ADVANC1-METH · ADVANced engineering of C1 metabolism towards METHanol-based sustainable biotechnology

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-11-01 → 2023-10-31
Финансиране от ЕС: 187 572 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Метанолът се изследва като алтернативна суровина за микробиално производство на химикали и горива. Това помага за намаляване на зависимостта от изкопаемите горива и освобождава земеделски земя, която иначе се използва за захарни култури.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

ADVANced engineering of C1 metabolism towards METHanol-based sustainable biotechnology

The project addresses pressing issues associated with climate change and fossil-fuel dependency. This challenge has compelled the EU to promptly swing into developing a biotechnological manufacturing platform to produce chemical commodities in a more sustainable manner. However, the major challenge for the development of bio-manufacturing is the need for sustainable, cheap, abundantly available feedstocks for microbial bio-production, which do not compete for food production. Bio-manufacturing platforms currently base their production mostly on plant-derived sugars or cellulosic material, which demand land use and thereby causing undesirable pressure on food security and biodiversity and hence, making EU framework unrealistic. Methanol is a promising, alternative one-carbon (C1) feedstock, which can be sustainably produced from;(i) waste resources or (ii) CO2 and renewable hydrogen. Being a liquid that can be easily transported, stored and utilized as a substrate for microbial production of valuable chemicals and fuels, utilizing methanol for bio-manufacturing could alleviate pressure on agricultural land, environmental concerns, and fossil-resource dependency, and hence, meet the EU policies on climate and energy framework of 2030 and beyond. Although the biological conversion of methanol to valuable products by natural methylotrophic bacteria and yeast is being studied. However, these natural methylotrophs still face various limitations for economically feasible bio-manufacturing, such as energy-inefficient methanol assimilation pathways, restricted product spectra as well as low product yields and rates, while their limited genetic toolboxes further prohibit their adoption for bio-manufacturing. Hence, the engineering of well-known biotechnological production microorganisms, such as the bacterium Escherichia coli, towards growth on methanol poses a pressing issue being addressed in this study. Through engineering of two synthetic methylotrophic routes in E. coli, namely, the Serine Threonine Cycle and the Reductive Glycine Pathway, the project developed an improved methanol assimilation in the system and hence, comprehensively supplement the wealth of knowledge towards methanol bio-economy.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Urgent global problems, including climate change and fossil-fuel dependence, force the EU to develop sustainable bio-basedchemical production platforms. However, current biotechnological feedstocks, such as plant-based sugars, suffer from serious limitations in terms of availability and sustainability. A very promising alternativeis the one-carbon (C1) feedstock methanol, which canbe produced in a sustainable manner from waste, or CO2 and renewable hydrogen. So far, the conversion of methanol to products is limited by the lack of suitable, efficientmicrobial cell factories. Hence, this project will engineer a highly efficient, synthetic methanol assimilation pathway into the well-known industrial host bacterium Escherichia coli. First, modular optimization will be performed for the bottleneck steps of enzymatic methanol conversion in the cell. Then, the overall strain performance will be optimized by rational, systems-wide targeting of the metabolic network and fast growing strains on methanol will be selected and studied. To achieve the objectives an innovative high-throughput genome editing approach will be employed. ADVANC1-METH is expected to yield a bacterial platform strain for efficient conversion of methanol to products, and industrially relevant IP and scientific knowledge will be effectively disseminated. The ADVANC1-METH project will aid the applicant to further extend his knowledge in the interdisciplinary fields of synthetic biology, bioinformatics, genetic and metabolic engineering, and complement his academic skills to fulfil the ambition to become an independent researcher. The implementation and training will be hosted at an institute with key expertise in bacterial and metabolic engineering. Within the ADVANC1-METH project videos and a board game will be developed to increase public engagement and knowledge on sustainable and methanol-based biotechnology, which are essential to realize a sustainable bio-based society.

Оригинален текст от CORDIS (на английски).

Участници

WAGENINGEN UNIVERSITY · WageningenКоординаторНидерландия