H2020Индивидуална стипендия2022–2024

DevBoundaries · Formation of tissue boundaries during zebrafish embryogenesis

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2022-09-01 → 2024-08-31
Финансиране от ЕС: 191 149 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Механизмите за формиране на граници между различните видове тъкани се изследват чрез ембрионите на зебрата. Разбирането на тези процеси помага да се обяснят тежки дефекти при развитието и състояния като рака и метастазите.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Formation of tissue boundaries during zebrafish embryogenesis

Separation of different cell types by tissue boundaries is a hallmark of all multi-cellular organisms. During animal development, boundaries between tissues not only arise to achieve physical and functional segregation between neighbouring groups of embryonic cells, but also serve as signalling centres that control downstream patterning events . Changes in the location or integrity of tissue boundaries often lead to severe defects in development, and the underlying cellular behaviours are also profoundly linked to disease states like cancer formation and metastasis . However, the mechanisms underlying tissue boundary formation are poorly understood: Successful formation of tissue boundaries requires a tight coordination between acquisition of cell fate and regulation of cellular properties responsible for morphogenesis, such as cell division, motility, polarity, and adhesion . It has been challenging to analyse how gene expression, cell lineages and cell movement coordinate in space and time. The overarching goal of this project is to probe the in vivo mechanisms of tissue boundary formation using the zebrafish embryonic shield region as a model system. I will generate a dynamic atlas characterizing the (1) cellular and (2) molecular basis of boundary formation, and (3) identify key regulators and their mechanism in cell differentiation and morphogenesis during boundary formation. This region has been of long-standing interest because it corresponds to the Mangold-Spemann organizer, but only now has it become tractable as a model system to study tissue boundary formation between multiple diverse cell types through the advent of technological revolutions in live imaging (Aim 1), spatial transcriptomics (Aim 2) and genome editing (Aim 3)

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

During animal development, boundaries need to be reliably established between cell types to guarantee the physical and functional integrity of tissues. This process requires delicate orchestration of cell proliferation, differentiation and migration, and defects in tissue boundary formation are often linked with abnormal cell behaviours in diseases. Genetic programs involved in cell signalling and cell sorting across tissue boundaries have been previously identified, yet we still lack a systematic understanding of the mechanisms underlying boundary formation, because it has been challenging to analyse the coordination of gene expression, cell lineages and cell movement in space and time. In this study, I will probe the in vivo mechanisms of tissue boundary formation using the zebrafish embryonic shield as a model system. The embryonic shield in zebrafish embryos is an evolutionarily conserved “organizer” structure that is critical for setting up the overall body plan of the embryos. The shield region contains overlapping progenitor cells that give rise to various tissue structures whose boundaries form and sharpen during gastrulation. However, the formation of shield progenitor cells into compartmentalized tissues has been a process that is poorly characterized, and the molecular and biophysical mechanisms that coordinate cell differentiation and morphogenesis to establish tissue boundaries remain elusive. I will utilize multi-disciplinary approaches that combine live imaging, spatial transcriptomics and genetic manipulation to determine how shield cells differentiate and generate tissue boundaries. I anticipate identifying cellular and genetic mechanisms controlling boundary formation, and provide a comprehensive and dynamic atlas detailing cell lineages, cell movement and gene expression of early embryogenesis in vertebrates.

Оригинален текст от CORDIS (на английски).

Участници

UNIVERSITAT BASEL · BaselКоординаторШвейцария