H2020Индивидуална стипендия2022–2024

Imagine · Imaging Functional Integration Of Newborn Neurons Into Neural Circuits Of The Axolotl Brain

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2022-03-01 → 2024-02-29
Финансиране от ЕС: 174 167 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Процесите на създаване и свързване на нови неврони в мозъка на аксолотла се проучват чрез изображения в реално време. Това помага да се разбере как новите клетки се интегрират в нервните мрежи, за да се възстанови функцията на мозъка след увреждане.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Imaging Functional Integration Of Newborn Neurons Into Neural Circuits Of The Axolotl Brain

In this project we have analyzed neurogenesis in the axolotl salamander brain during homeostasis and regeneration and developed methodology to analyse neuronal projections and connections. Neurogenesis, the generation of new neurons from stem or progenitor cells, occurs universally during embryonic development across all animals. Following embryonic development, certain species maintain ongoing neurogenesis into adulthood to varying degrees. In humans, regions like the hippocampus exhibit continuous adult neurogenesis, producing approximately 700 new neurons daily. Conversely, species like teleost fish and amphibians experience lifelong growth, necessitating constant cell generation in multiple brain areas. In zebrafish and axolotl, continual neurogenesis is observed in various brain regions, including the telencephalon, mesencephalon, and olfactory bulb. Notably, these species possess the unique ability to regenerate lost neurons after injury, a feature distinct from mammals, where neuronal death typically results in glial cell hypertrophy and scar formation, impeding neuronal regeneration. Both adult neurogenesis and neural regeneration face a crucial challenge: integrating newborn cells into existing neural networks. Maintaining and restoring neural connectivity and circuit function is vital for controlling animal behavior and regulating physiology. Thus, newborn cells must be appropriately integrated and connected to target cells to ensure the functionality of the neural network is preserved or restored. The objectives of this Marie Skłodowska Curie Action (MSCA) have been to (a) Develop in vivo imaging of neurogenesis during homeostasis and regeneration of the axolotl brain, to (b) set up in vivo imaging of functional neural circuits, their growth and regeneration in the axolotl and (c) develop methods to visualize functional connections of neurons. We have been successful in developing a variety of methods visualize functional connections of neurons which will in the future be useful to determine neuronal circuit recovery after brain regeneration in the axolotl.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Post embryonic growth and regeneration require new cells to integrate into the network of existing cells. In the case of the brain it is extremely important to maintain and restore circuit function, such that behaviors can be executed correctly. To study such processes a system that fulfills both post-embryonic growth as well as regeneration is needed. Furthermore, since neural circuit functions are highly dynamic the need for in vivo approaches is evident. In mammalian systems where neurogenesis occurs throughout life in restricted brain regions such approaches are technically challenging.Here, I propose to use the axolotl (Ambystoma mexicanum) brain to understand the dynamic remodeling of neural circuits through the addition of newborn neurons during post-embryonic growth and regeneration. I will establish a high resolution intra-vital in vivo imaging approach to address the dynamics of neurogenesis and remodeling of neural circuits in the telencephalon and the optic tectum. Using this setup in combination with genetically encoded calcium indicators I will generate activity maps of these regions in the presence or absence of defined stimuli and address their functional remodeling during growth and their restoration after injury. Furthermore, I will develop and adapt methods to visualize the functional connections of neurons in axolotl. Together, this work will provide fundamental insights into the functional maintenance and regeneration of neural circuits and the dynamics of how new neurons integrate in vivo which has not yet been achieved in any vertebrate system.

Оригинален текст от CORDIS (на английски).

Участници

FORSCHUNGSINSTITUT FUR MOLEKULARE PATHOLOGIE GESELLSCHAFT MBH · WienКоординаторАвстрия