H2020Индивидуална стипендия2021–2023

PlantSeeFe · Plants seeking iron: unraveling the molecular mechanisms involved in iron-mobilizing coumarins distribution and trafficking

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-09-01 → 2023-08-31
Финансиране от ЕС: 196 708 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Молекулярните механизми, чрез които растенията като Arabidopsis thaliana разпределят и отделят кумарини, помагат за усвояването на желязо от почвата. Разбирането на този процес може да помогне за повишаване на съдържанието на желязо в културите, за да се подпомогне лечението на анемия.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Plants seeking iron: unraveling the molecular mechanisms involved in iron-mobilizing coumarins distribution and trafficking

Fe is an essential metal for a wide range of physiological events in many organisms, since it serves as a central component of various types of metalloproteins. A deficiency of Fe nutrition often causes developmental and growth issues for both animals and plants. This project, by studying the Fe acquisition by plants might help improving Fe content in crops and thus provide a sustainable wat to provide Fe to people who are suffering from Fe-dependent anaemia all over the world (about one billion persons). Fe is the 4th most abundant metal on earth; however, the majority of Fe existing in natural soils is non-bioavailable as it is present in the form insoluble oxyhydroxides, in particular under aerobic and/or alkaline conditions. To cope with these harsh environments, plants have evolved sophisticated mechanisms to efficiently absorb Fe. Recent studies reported coumarin secretion from roots to play an important role in this process in non-gramineous plants, like Brassica and legume species. In particular, Fe-mobilizing coumarins (FMCs), mainly fraxetin and sideretin, are major compounds involved in Fe acquisition because of their chelation activity against trivalent metals such as ferric Fe (Fe3+). Interestingly, fraxetin was recently revealed to be accumulated in specific cell types within roots (i.e. epidermis, cortex and endodermis cell layers) using the model plant Arabidopsis thaliana. This suggested that the formation of uneven distribution of coumarins within roots should be essential for optimizing their secretion and Fe acquisition. However, the molecular mechanisms underlying FMCs transport remained largely unknown. The overall objective of this project was to decipher how coumarin distribution and secretion are controlled in Arabidopsis roots in order to acquire Fe from soils. The Fellow recently isolated from Arabidopsis, by analysing genome-wide gene expression of Arabidopsis roots exposed to Fe deficient conditions, Coumarin Import Transporter (CIT) genes. Within the frame of this project, the Fellow aimed at answering the following three questions (1) Which coumarins (aglycones or glycosides) are the transport substrates of CITs? (2) Where (tissue, cell or organelle) do CITs mediate coumarin transport activity? (3) How are CIT transport activities modulated?

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Iron (Fe) is an essential micronutrient for the productivity of crops and the quality of their derived products. Fe deficiency-induced anemia affects billions of people worldwide. This could be mitigated by improving the capacity of plants to absorb and store Fe, which will help to provide adequate dietary Fe to the growing global population. Decrypting the molecular mechanisms plants have evolved to acquire Fe from the soil is essential for reaching this objective. Recently, the secretion in the soil of root-borne coumarins has emerged as a key factor affecting Fe uptake in non-grass species. However, the mechanisms that modulate coumarin storage and trafficking within plant roots are not well understood. The candidate has coupled microarray data mining with in vivo coumarin transport assays and identified four novel Arabidopsis coumarin transporters, namely NPF7.2, NPF2.3, NPF2.4 and NPF2.5. The objective of this project is to determine the precise function of each of these four transporters in coumarin trafficking and plant Fe nutrition. In work package 1 (WP1), the coumarin transport activities of NPF candidates will be examined in detail using yeast cells and Xenopus oocytes. Moreover, we will generate single (insertion/transposon lines) and multiple (CRISPR/Cas9) mutants in order to precisely define the role of each NPF in plant roots. Next, in vivo imaging of coumarin and NFP candidate protein localization will be conducted to ascertain the role of each candidate in the spatiotemporal distribution of coumarins within the roots (WP2). Finally, the posttranslational regulation of the NPF candidates will be investigated using proteomic approaches (WP3). The proposed project is both feasible and appropriate for a Marie Skłodowska-Curie Individual Fellowship, since the expected outcomes are innovative and will generate knowledge for both fundamental and industrial research, while the candidate will improve his skills and knowledge in a specialized research area.

Оригинален текст от CORDIS (на английски).

Участници

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT · ParisКоординаторФранция