H2020Индивидуална стипендия2022–2024

Virus RePro IEL · Reprogramming of IELs at the intestinal epithelial barrier during virus infection

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2022-01-01 → 2024-02-28
Финансиране от ЕС: 147 464 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Т-клетките в чревната бариера се проучват чрез наблюдение на инфектирани мишки, за да се разбере как вирусът MCMV променя поведението им. Това помага за разясняване на патогенезата на вирусните инфекции и търсенето на нови диагностични методи и терапевтични цели.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Reprogramming of IELs at the intestinal epithelial barrier during virus infection

This MSCA offers a chance to explore how MCMV shapes CD8 T cells at the intestinal epithelial barrier and the processes involved in this modulation within mucosal defenses. The relationship between T cells and viruses presents a major challenge in contemporary medicine. Deepening our knowledge of viral interactions with T cells at the intestinal barrier is essential for grasping the pathogenesis of viral infections at mucosal barriers. The data we gathered could lead to new diagnostic methods and therapeutic targets for intestinal mucosal issues, which are of considerable health importance. We sought to explore the behavior of intraepithelial lymphocytes (IELs) in infected and non-infected mice and their response to different intestinal challenges, such as DSS-induced colitis, antibiotic treatment, and a high-fat diet. Our primary goal was to identify the potential mechanisms by which the virus influences IELs at the intestinal epithelial barrier in infected mice and to compare this with the impact of virus infection on circulating CD8 T cells in the spleen.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Intestinal epithelial resident CD8+ T lymphocytes, such as intraepithelial lymphocytes (IELs), are located at epithelial barriers. They play a key role in tissue protection by promoting the elimination of infected cells, while producing antimicrobial factors and epithelial growth factor. Due to the positioning of IELs underneath the single epithelial layer and their potential involvement in modulating intestinal pathology, the activation status of IELs is intensively studied. Viruses modify CD8 T cells in many ways. Murine cytomegalovirus (MCMV) belongs to β-herpesviruses and is often used as an animal model to study the pathogenesis of human cytomegalovirus (HCMV). MCMV specific resident memory CD8 T cells has been described in intestine. Memory CD8 T-cell in the gut epithelium induced by persistent viruses such as MCMV has a distinct quality from both conventional memory and “inflationary” memory CD8 T cells, which may be relevant to protection against mucosal infections. The outcome for patients with HCMV reactivation appears worse than for patients without reactivation. It is not absolutely clear whether HCMV is a contributor or a bystander cause of inflammatory bowel disease (IBD). In this proposal we aim to define the possible mechanisms by which virus impact IELs at the intestinal epithelial barrier in comparison to virus impact on circulating CD8 , and how they then behave in different challenges to which intestine could be exposed such as DSS triggered colitis, antibiotic treatment and high fat diet. For this, we will use sequencing approaches and data analysis of gene in different conditions in IELs. How the IELs “reprogram” their gene composition due to combination of the challenges will be our main goal additionally to classical approaches to with the immunological and biochemistry techniques. These findings can potentially lead to new diagnostic methods and therapeutic targets in mucosal conditions that are major public health burden.

Оригинален текст от CORDIS (на английски).

Участници

SVEUCILISTE U RIJECI, MEDICINSKI FAKULTET · RijekaКоординаторХърватия