H2020Индивидуална стипендия2021–2023

RET-TRAF · RET Trafficking

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-09-01 → 2023-08-31
Финансиране от ЕС: 203 852 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Рецепторът RET и неговото движение в клетката се проучват чрез специални светлинни инхибитори, за да се види как влияе върху миграцията на клетките. Това помага да се разбере връзката между ензимна активност и развитието на ракови клетки.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

RET Trafficking

The transmembrane receptor tyrosine kinase, REarranged during transfection (RET), crucially influences the development of the enteric nervous system, kidneys, and spermatogenesis. Two isoforms, RET9 and RET51, arise from alternative splicing, exhibiting unique C-terminal amino acids. RET trafficking regulates its oncogenic potential in cancer cells by coordinating signal location and duration. This project aims to use photoresponsive RET inhibitors (PRETIs) in conjunction with imaging to explore RET's role in cell motility, migration, and invasion with unprecedented spatiotemporal resolution. The project can be divided into two interconnected Research Objectives (ROs): designing PRETIs (RO1) and developing an experimental approach for real-time RET trafficking monitoring using light control (RO2). The project's overarching goal is to understand the relationship between intracellular enzymatic activity and RET trafficking.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Protein kinases play a critical role in a number of cellular processes, including cell proliferation, differentiation and apoptosis. The REarranged during Transfection (RET) receptor tyrosine kinase plays important roles in regulating cellular proliferation, migration, and survival in the normal development of neural crest derived tissues. Furthermore, aberrant activation of RET, through oncogenic mutations or overexpression, can contribute to tumourigenesis, regional invasion, and metastasis of several human cancers. RET relocalisation plays a role in cancer invasion and metastasis. Unfortunately, the detailed understanding of RET's dynamic function and the importance of quantitative, spatial and time-dependent parameters regarding RET trafficking is lacking. As such, the ability to manipulate RET activity using light would result in temporal control of enzymatic activity, thus serving as a valuable approach to probe the RET trafficking, further our understanding of cell movement and invasion. Cells will be exposed to photoresponsive RET inhibitors that can be turned on and off with light. Their effects on RET trafficking will be monitored by imaging. The results of these studies will provide new insights into intracellular RET trafficking and its role in cancer, ultimately leading to new therapeutic targets and improved disease management.

Оригинален текст от CORDIS (на английски).

Участници

GOETEBORGS UNIVERSITET · GoeteborgКоординаторШвеция