H2020Индивидуална стипендия2022–2025

NELLAFLOW · New generation of low-cost Lateral Flow Assays (LFAs) coupled with paper-based electrical gas sensors: An application study for the rapid determination of Salmonella in food

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2022-02-01 → 2025-01-31
Финансиране от ЕС: 319 401 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Нови тестове за бързо откриване на салмонела в храните се разработват чрез комбиниране на хартиени сензори и електрическо измерване. Това помага за по-бързо и точно определяне на количеството патогени, вместо стандартните бавни лабораторни анализи.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

New generation of low-cost Lateral Flow Assays (LFAs) coupled with paper-based electrical gas sensors: An application study for the rapid determination of Salmonella in food

Over 420,000 people die each year due to contaminated foodstuffs, yet conventional methods for determining pathogens in food require lengthy procedures, lab-based facilities and trained staff. Lateral Flow Assays (LFAs) are a powerful alternative method for pathogen testing because they are rapid, simple to use, and amenable to Point-of-Need. Current LFAs available in the market only provide binary (yes/no) or semiquantitative responses, lacking the rapidness and sensitivity required for food monitoring due to the limitations of the colorimetric transduction. Many efforts are focused on addressing the lack of quantification on this type of devices by combining LFAs and electrical detection, but the integration of metallic electrodes on nitrocellulose membranes remains a bottleneck. The NELLAFLOW project sought to develop a rapid, quantitative test for the determination of pathogens in food by the integration of paper-based electrical gas sensors (PEGS) as detectors into LFAs. PEGS have already proven to be highly sensitive towards water-soluble gases. They exploit the intrinsic hygroscopic characteristics of cellulose paper and the changes in its ionic conductance in the presence of water-soluble gases. To fulfil the overall objective, the following technological goals were approached: design and optimization of PEGS for integration into LFA systems; design of an immunoassay for pathogen determination using gas detection; application of the gas sensor-based LFA prototype for real sample testing. Through a multidisciplinary approach (biomolecular engineering, analytical chemistry, printed electronics, microbiology) NELLAFLOW aimed to tackle a key problem in the food industry with an innovative, versatile technology that can be easily transferred to other application fields.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Over 420,000 people die each year due to contaminated foodstuffs, with Salmonella identified as one of the key global causes. Yet conventional methods for the determination of Salmonella in food require several enrichment and subculture steps performed by trained staff at lab-based facilities, taking up to 5 days to obtain a result.Lateral Flow Assays (LFAs) have emerged as a powerful alternative method for Salmonella testing because they are rapid, simple-to-use and amenable for Point-of-Need (PoN). However, current LFAs available in the market only provide binary (yes/no) or semiquantitative responses, lacking the rapidness and sensitivity required for food monitoring due to the limitations of the colorimetric transduction.By the integration of paper-based electrical gas sensors (PEGS) as detectors into Lateral Flow Assays, NELLAFLOW will address the challenge of quantitative determination of Salmonella in food without the requirement of long enrichment steps. PEGS have already proven to be highly sensitive towards water-soluble gases determination. They exploit the intrinsic hygroscopic characteristics of cellulose paper and the changes in its ionic conductance in the presence of water-soluble gases. The incorporation of aptamers as biological recognition elements in the PEGS-LFA platform will improve the implementation of the prototype for on-site monitoring as they are low-cost, highly specific, and stable at room temperature. Through a multidisciplinary approach (biomolecular engineering, analytical chemistry, printed electronics, microbiology) NELLAFLOW will tackle a problem in the food industry with an innovative versatile technology that can be easily transferred to other application fields.By combining academic and non-academic training, NELLAFLOW will broaden the Fellow expertise through new technical and management skills that will complement her previous experience while delivering high impact results and transfer of knowledge.

Оригинален текст от CORDIS (на английски).

Участници

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE · LondonКоординаторОбединеното кралство