H2020Индивидуална стипендия2021–2023

FuelingLPMO · LPMO regulation by (poly)phenolics-active enzymes in Ascomycota

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-10-01 → 2023-09-30
Финансиране от ЕС: 207 312 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Ензимите LPMO при гъбите разграждат растителна биомаса, като например дървесина и земеделски отпадъци. По-доброто разбиране на тези процеси помага за създаването на ефективни технологии за производство на биогорива и екологични химикали.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

LPMO regulation by (poly)phenolics-active enzymes in Ascomycota

This project addresses the challenge of converting lignocellulosic biomass, e.g. non-edible agro-industrial wastes and wood, into sustainable biofuels, green chemicals and materials via enzyme technology. A class of microbial enzymes termed lytic polysaccharide monooxygenases (LPMOs) plays a central role in the depolymerization of polysaccharides that compose lignocellulosic biomass, such as cellulose, in Nature and in industrial settings. However, the exact mechanism employed by LPMOs to depolymerize plant polysaccharides is still not fully understood. New knowledge on the LPMO reaction mechanisms and its interplay with other oxidative enzymes involved in the degradation of plant biomass is required to provide the basis for the development of efficient enzyme technologies for lignocellulosic biomass refining. Enzyme-based biorefineries will have important role in the transition from a fossil-based economy to a more sustainable biomass-based economy. The overall objective of this research project is to contribute with a more detailed understanding of the network of enzyme-catalyzed redox reactions involved in lignocellulose degradation by fungi, building on the hypothesis that such understanding can be used to design powerful enzyme systems for efficient biomass conversion into bioproducts.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Lytic polysaccharide monooxygenases (LPMOs) are oxidative enzymes employed by fungi to depolymerize cellulose and thus of great academic and industrial (e.g. biorefinery) interest. The exact way or ways natural electron and H2O2 supply occurs to LPMOs during natural fungal LPMO action (and activation) remains unclear. Recent evidence suggests that LPMOs benefit from two oxidative enzymes (i.e. polyphenol oxidases (PPOs) and laccases) that are active on (poly)phenolic compounds. This gives rise to a new paradigm that places lignin as a protagonist in the activation of cellulose degradation, and not simply an obstacle to biomass decay. FuelingLPMO aims to investigate if and how a novel group of uncharacterized flavoenzymes, which I and other researchers have independenlty reported to be commonly co-secreted with AA9 LPMOs by ascomycetes during lignocellulose degradation, can regulate LPMO activity by modifying lignin-derived compounds, as well as deepen the current understanding on the mechanisms involved in LPMO activation by PPOs and laccases. We will use a combination of genome database mining, proteomics analysis and enzyme synergy characterization to investigate the co-occurence of genes encoding for AA9 LPMOs and these putative LPMO regulatory enzymes among Ascomycota, validate their co-secretion during lignocellulose degradation and test their hypothesized role in the regulation of cellulose degradation by AA9 LPMOs in vitro. The project will form a collaboration between the two research laboratories that made the ground-breaking discoveries of the PPO-mediated and laccase-mediated activation of LPMOs and exploit the multi-/interdisciplinary nature of their studies. Our findings will contribute to a more detailed understanding of the network of redox reactions involved in lignocellulose degradation by ascomycetes, building on the hypothesis that such understanding can be used to design powerful enzyme systems for efficient biomass conversion into bioproducts.

Оригинален текст от CORDIS (на английски).

Участници

DANMARKS TEKNISKE UNIVERSITET · Kongens LyngbyКоординаторДания