RNAhello · Exosome inspired RNA communication between HiErarchicaL poLymer prOtocells
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2021-06-01 → 2023-05-31
- Финансиране от ЕС
- 175 572 €
- Участници
- 1
- Схема
- MSCA-IF
Линиите свързват координатора с партньорите.
Накратко на български
Изследва се преносът на RNA между изкуствени и живи клетки чрез специални полимерни капсули, които реагират на UV светлина. Това помага за по-доброто разбиране на клетъчните процеси и развитието на бъдещи терапевтични технологии.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
Exosome inspired RNA communication between HiErarchicaL poLymer prOtocells
The project sought to mimic biological communication and information transfer in artificial multicellular systems. Development of artificial cell systems has the potential to increase our understanding of cellular processes and biological functions, produce therapeutic technologies for advancement of global healthcare, and give rise to further understanding into how life evolved on earth. Firstly, the effect of coacervate artificial cell constituents on enzymatic activity was investigated. This project has resulted in a manuscript which is ready to be submitted for publication. Secondly, in order to achieve RNA communication between artificial cells and living cells, a UV responsive polymersome system was developed as a shuttling vehicle. siRNA transfer from coacervates to HELA cells was successfully observed, and further experiments are investigating gene knockdown in living cells, based on this exosome inspired system. The development of protocell based communication with exosome-inspired RNA containing polymersome transfer, is a significant advancement in the artificial cell and systems chemistry field. This second project has also resulted in a manuscript which is almost ready to be submitted for publication. The project has concluded that RNA transfer between artificial cells and living cells is possible when encapsulating the RNA in UV responsive polymersomes. The results pave the way for artificial cells to be used as therapeutic devices in the future, due to protection of typically unstable RNA molecules inside polymersomes.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The mimicking of cellular communication pathways and genetic information transfer in synthetic systems is an important target for researchers. These artificial cell systems have the potential to increase understanding of biological and cellular processes, while also having potential applications in healthcare technologies. This project will involve the investigation of exciting new stimuli responsive and highly tuneable hierarchical polymer protocells, and their application in RNA-based communication. Previous generations of polymer protocells have been shown to allow diffusion based communication with small molecules and naked DNA strands – the project will expand upon this by investigating polymersomes as mRNA shuttles between distinct protocell populations. This exosome inspired system will allow the typically unstable mRNA molecules to be protected from degradation in the external medium, thus opening the door to much more lifelike systems, and possible therapeutic applications. The proposed research project is strongly multidisciplinary, and involves a combination of polymer chemistry, nanomaterials engineering, cell biology, and imaging techniques. RNAhello includes both the transfer of knowledge to the host institution and the training of the candidate in new advanced techniques. Results have the potential to increase understanding of biological mechanisms in relation to the complex hurdle of the nucleic acid based transfer, whilst also providing innovative platform material for treatment of disease. Additional project goals include: engaging with the non-scientific public through outreach methods; gaining of further commercial training for potential exploitation of research; presentation of research results at international conferences; and the preparation of additional funding proposals.
Оригинален текст от CORDIS (на английски).
Участници
- TECHNISCHE UNIVERSITEIT EINDHOVEN · EindhovenКоординаторНидерландия
Връзки
Данни: CORDIS, © Европейски съюз