NemHostRangeParadox · A nematode host-range paradox: how can one of the apparently most specialised obligate biotrophic plant-parasites also have the largest host rangeNemHostRangeParadoxA nematode h
„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“
- Период
- 2021-10-01 → 2023-09-30
- Финансиране от ЕС
- 224 934 €
- Участници
- 1
- Схема
- MSCA-IF
Линиите свързват координатора с партньорите.
Накратко на български
Галеровидните нематоди (Meloidogyne incognita) се изследват, за да се разбере как един специализиран паразит атакува над 3000 различни растения, включително зеленчуци и зърнени. Анализът на техните гени помага да се разберат механизмите, чрез които тези вредители препрограмират растителните клетки за собствено хранене.
Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.
Резултати накратко
A nematode host-range paradox: how can one of the apparently most specialised obligate biotrophic plant-parasites also have the largest host rangeNemHostRangeParadoxA nematode h
The root-knot nematode (Meloidogyne incognita) is one of the most important pests worldwide, especially in tropical and subtropical areas of the globe. This single nematode species is able to parasite more than 3,000 different plant hosts including fruits, vegetables and cereals. It exploits the plant resources by using very specialized mechanisms of parasitism. It reprograms the plant cell using small secreted molecules (termed ‘effectors’), culminating in the development of a new pseudo-organ (termed ‘giant cells’), which presents the sole purpose of nourishing the nematode. Although remarkable, it is currently not understood the mechanism underlying this paradox – a specialised parasite presenting the broadest host range of all biotrophic pathogens known. The HostRangeParadox project aimed to build a holistic view of M. incognita parasitism by analysing the transcriptome profile of the root-knot nematode on ten different host plants. We aimed to identify root-knot nematode’s genes (and consequently effectors) expressed across several hosts or on a specific host. Also, we addressed the host side of the interaction, aiming to identify possible targets (such as metabolic pathways or plant genes) of M. incognita. Finally, our last objective was to confirm our hypothesis: the necessity of some effectors to hosts or in particular interactions and confirm the importance of certain targets in the host x M. incognita interaction.
Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз
Цел на проекта
The root-knot nematode (Meloidogyne incognita) is an obligate biotrophic pathogen with a remarkable ability to deliver “effector” molecules into its host plant, allowing it to reproducibly and exquisitely alter plant immunity, development, and physiology. Paradoxically, this nematode also has the widest host range of any obligate biotroph: parasitizing more than 3,000 different species. I hypothesize that the polyploid nature of its genome allows it to harbor an “effector” repertoire that not only allows for this broad host range, but its differentially deployed across its host range. To address this hypothesis, we have carefully pre-selected plant species across the botanical orders covering the main groups across the phylum. We will infect each species with M. incognita and, in a “trans-kingdom linked transcriptome” we will analysed nematode gene expression, and plant gene expression, at the same time in each susceptible interaction. These data will allow us to determine: 1) Is there a core set of effectors that are invariantly deployed, independent of host, and/or are subsets “chosen” for particular hosts or groups of hosts?; 2) Are there conserved plant genes manipulated by M. incognita during the infection?; and 3) Finally, are some plant or nematode genes required for specific interactions or group of interactions? These data will allow us to address a long-standing question in the field, and I believe understanding how M. incognita can infect such a broad host range may be a key to future control.
Оригинален текст от CORDIS (на английски).
Участници
- THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE · CAMBRIDGEКоординаторОбединеното кралство
Връзки
Данни: CORDIS, © Европейски съюз