H2020Индивидуална стипендия2021–2023

Multi-IF · Multicolor Fluorescent Ultrashort Carbon Nanotubes for Multiplexed immunofluorescence in Cancer Diagnostics

„Хоризонт 2020“ — Действия „Мария Склодовска-Кюри“

Период: 2021-11-01 → 2023-10-31
Финансиране от ЕС: 184 708 €
Участници: 1
Схема: MSCA-IF

Линиите свързват координатора с партньорите.

Накратко на български

Въглеродни нанотръби се тестват като нови светещи маркери за откриване на специфични протеини в тъкани от черен дроб. Това помага за по-точна диагностика на рака, като преодолява проблемите с бързото избледняване на стандартните багрила при микроскопските изследвания.

Този кратък обзор е генериран от изкуствен интелект

Кратко обяснение, генерирано от езиков модел по текста на CORDIS. Оригиналът е по-долу.

Резултати накратко

Multicolor Fluorescent Ultrashort Carbon Nanotubes for Multiplexed immunofluorescence in Cancer Diagnostics

Heterogeneity is a common indication of cancer. In order to fight cancer pathology, a successful and more effective diagnosis is the essential criteria to adopt optimum treatment and subsequent clinical management. Histopathology, based upon immunohistochemistry (IHC), is the well-established and classical tool of choice for diagnosis of many cancers. However, this technique needs the usage of a lot of tissue biopsy and commonly used labels organic fluorophores suffer from severe photobleaching and preventing simultaneous multiplexed IHC/immunofluorescence due to spectral overlaps. “Multi-IF” proposed to investigate an innovative approach combining nanoscience, click chemistry, surface (bio-)functionalization and fluorescence microscopy for the advancement of cancer histopathology with the aim to divulge the expression level of proteins of interest. Our overall objective was the proof-of-concept that single walled carbon nanotubes (SWCNTs) can be used as novel fluorescent immuno-probes and thus open new strategies for multiplexed immunofluorescence. The use of SWCNTs finds its roots in coordinator’s expertise on optical microscopy due to their strong photoluminescence (PL) in short wave infrared (SWIR) window with high brightness and photostability. This project involved in the preparation and characterization of novel immuno-labels using ultrashort carbon nanotubes (usCNTs), the development of a microscope dedicated to SWIR single nano-labels imaging and the proof-of-concept of this on liver tissue for cancer diagnostic.

Текст от CORDIS, на английски · Данни: CORDIS, © Европейски съюз

Цел на проекта

Multi-IF proposes a fully innovative approach combining nanoscience, fluorescence microscopy and cancer histopathology to achieve optically multiplexed immunofluorescence for cancer diagnostic. At present, when examining cancer tissue, biomarker analyses are sequential and require several biopsy slices implying a lot of (not always available) biological material. Testing several biomarkers on a single tissue biopsy slice in an efficient, quantitative and reproducible way, would be crucial but not currently available at hospital. This project will involve an innovative technology based on single-walled carbon nanotubes to create a library of novel multicolor immuno-labels. More precisely, this will employ sp3 defects implanted fluorescent ultrashort nanotubes together with the development of a dedicated NIR microscope for multiplexed immunofluorescence on a single biopsy and the proof-of-concept on liver cancer tissue. The applicant will bring strong expertise in chemical synthesis, bioprobe spectroscopy and time-resolved fluorescence microscopy applied to the study of complex and heterogeneous bio-molecular organization (2D/3D cells, proteins and peptides). Concomitantly, he will gain a high-level hands-on training on carbon nanotube optics, near-infrared advanced spectroscopy, custom-built microscopy techniques, cancer tissue model and imaging analysis. To achieve this objective, the project will be performed at one of the leading French research institution, IOGS-LP2N at the CNRS/Univ. Bordeaux, with well-identified work packages to ensure efficient management, career development and training, science development, communication and technology transfer to pathologists. It will allow the applicant to reach high level of professional maturity to develop independent research career. The fellow’s expertise, privileged environment of the hosting lab, the mentoring, the infrastructure & relevant facilities will guarantee the successful execution of Multi-IF goals.

Оригинален текст от CORDIS (на английски).

Участници

INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE IOTA - SUPOPTIQUE · PalaiseauКоординаторФранция